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文献和实验Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris
The full-length and truncated forms of recombinant envelope (E) glycoprotein from Dengue virus type 1, Singapore strain S275/90 were expressed in the yeast, Pichia pastoris , using a secretory vector. A truncated form of the E protein
Scale-Up of recombinant virus and protein production in Stirred-Tank reactors
culture using these fermentors for large-scale productions (i.e., New Brunswick Scientific’s CelliGen, New Brunswick, NJ; Alfa-Laval’s Chemap, Mannedorf, Switzerland). Stirred-tank reactors (STR) are well known and characterized (as well as airlift
Strategies to Optimize Protein Expression in E. coli
depicting the critical factors to consider, common obstacles, and potential solutions for each stage of protein expression in E. coli . The left column lists the major steps of recombinant protein expression with key variables to consider. The middle column
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