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文献和实验Western Blotting with Biotinylated Antibodies
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
Western Blotting with Biotinylated Antibodies
Western Blotting with Biotinylated Antibodies SAMPLE PREPARATION For Protein Concentration Determination of Cell Culture Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline
BioID: A Screen for Protein‐Protein Interactions
expressing cells. (4 ) Induce biotinylation with excess biotin prior to (5 ) cell lysis and protein denaturation. (6 ) Perform biotin‐affinity capture of biotinylated proteins. (7 ) Reserve 10% of the sample for streptavidin immunoblot analysis. Use
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