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文献和实验Use of Primary Human Cells in High-Throughput Screens
compounds that mediate a change in a defined physiological end point as a consequence of compound activity in human primary cells. Rather than screening at a defined molecular target, such “phenotypic” screens permit the identification of compounds that act
Primary MicroRNA Processing Assay Reconstituted Using Recombinant Drosha and DGCR8
protein DiGeorge critical region 8 (DGCR8). DGCR8 has been shown to tightly bind an Fe(III) heme cofactor, which activates its pri-miRNA processing activity. Here we describe how to reconstitute pri-miRNA processing using recombinant human Drosha and DGCR8
Protein Engineering of Interferon Alphas
differences in these proteins may be responsible for a significant variety of biological actions. Understanding the structure and function of human IFN-α is very important. Recombinant techniques are important tools for the production and modification of IFN
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