Biotinylated Recombinant SARS-

High-Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant S1P Receptor Subtypes

Intracellular Ca2+ mobilization is a useful readout to screen for agonists or antagonists of G-protein �coupled receptors (GPCRs). Here, we describe methods to conduct high-throughput screening of stably or transiently transfected HTC4 cells

Cell Death Differ：中大五院黄曦团队发现新冠病毒引起肺损伤的分子机制

病理发生。然而，SARS-CoV-2 感染引起肺细胞凋亡的机制仍不清楚。 2022 年 1 月 13 日，中山大学附属第五医院黄曦课题组在 Cell death & differentiation 上发表了题为 SARS-CoV-2 membrane protein causes the mitochondrial apoptosis and pulmonary edema via targeting BOK 研究性论文。该研究证实了病毒膜（M）蛋白会通过靶向 BOK 分子，介导肺

BioID: A Screen for Protein‐Protein Interactions

expressing cells. (4 ) Induce biotinylation with excess biotin prior to (5 ) cell lysis and protein denaturation. (6 ) Perform biotin‐affinity capture of biotinylated proteins. (7 ) Reserve 10% of the sample for streptavidin immunoblot analysis. Use