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文献和实验Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
. Elution of the complexes by competition with D-biotin is efficient and can be done in a variety of buffer conditions allowing biochemical active complexes to be preserved. For more theoretical background and a protocol for purification of recombinant
Non-fluorescent protein/RNA double-labeling
of PBTH. In situ HYBRIDIZATION : 1. Fix the embryos for 20 minutes with 5% formaldehyde in PBT (PBT= filtered 1 X PBS with 0.1% Tween-20) at room temperature. 2. Wash 3 X 5 minutes with PBT. 3. Incubate the embryos with 50 ug/ml
In vitro site-specific mutagenesis
to evaluate the contribution of a specific amino acid to the biological function of a protein. A popular general approach involves cloning the gene or cDNA into an M13 or phagemid vector which permits recovery of single-stranded recombinant DNA (
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