Recombinant Mouse CD28 Protein

用 CD3/CD28 做好 T 细胞激活与扩增

分别利用 CD3 与 CD28 的功能学抗体，以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤： 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体（克隆号：145-2C11）稀释至 5μg/ml，稀释后的抗体加入到 24 孔板中，每孔 400μl，4°C 包被过夜。（注：板子在加入抗体之前，先用无菌 PBS 润洗 2-3 遍，避免干孔）； 1.2 小鼠脾脏淋巴细胞分离（次日） 1. 将 70μm

Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells

RPMI Medium 1640 with 2 mM L-Glutamin, 10% FCS/FBS and 100 U/ml penicillin/streptomycin can be used, or an equivalent culture medium. Heat inactivated Fetal Calf Serum (FCS). Recombinant mouse IL-2 (human IL-2 can also be used).  

Recombinant Protein Production in Yeasts

Recombinant protein production is a multibillion-dollar market. The development of a new product begins with the choice of a production host. While one single perfect host for every protein does not exist, several expression systems ranging