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- CAS号：
446-72-0
- 规格：
1mLx10mM(inDMSO)/50mg/100mg/500mg
| 规格： | 1mLx10mM(inDMSO) | 产品价格： | ￥368.0 |
|---|---|---|---|
| 规格： | 50mg | 产品价格： | ￥198.0 |
| 规格： | 100mg | 产品价格： | ￥333.0 |
| 规格： | 500mg | 产品价格： | ￥591.0 |
Product Introduction
Bioactivity
| 名称 | Genistein |
| 描述 | Genistein (NPI 031L) is a naturally occurring soy isoflavone, a multi-targeted tyrosine kinase inhibitor. Genistein has antitumor, antioxidant, and anthelmintic properties, and also produces estrogen-like effects in the body. |
| 细胞实验 | Genistein is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. The IC50 values for Genistein are determined by the MTT assay. Briefly, the MTT assay is a colorimetric assay that is based on the ability of living but not dead cells to reduce a tetrazolium-based compound to a blue formazan product. The formazan crystals are solubilized in DMSO, and the absorbance is measured at 540 nm. The absorbance at 540 nm is proportional to the number of viable cells. The lC50 values obtained with the MTT assay are compared with the lC50 values obtained by counting viable cells using trypan blue dye exclusion and by tritiated thymidine incorporation into DNA[1]. |
| 激酶实验 | Purified FXa is obtained after activation with Russell's viper venom followed by affinity chromatography. The resulting FXa is > 95% pure as judged by sodium dodecylsulfate polyacrylamide gel electrophoresis. The substrate affinity values for FXa, expressed as the Michaelis-Menten-Henri constant (Km), for human, rabbit, rat and dog FXa are determined using the chromogenic substrate S-2765, and are 36, 60, 240 and 70 μM, respectively. The substrate hydrolysis is monitored by measuring absorbance at 405 nm at 25°C for up to 30 min using a SpectraMax 384 Plus plate reader and SoftMax. FXa activity for each substrate and inhibitor concentration pair is determined in duplicate. The Ki values are calculated by non-linear least-squares fitting of the steady-state substrate hydrolysis rates to the equation for competitive inhibition (Equation 1) using GRAFIT, where v equals reactions velocity in OD min?1, Vmax equals maxiumum reaction velocity, S equals substrate concentration, and I equals inhibitor concentration. |
| 体外活性 | 方法: 乳腺癌细胞 MCF-7 用 Genistein (10-200 µM) 处理 24-48 h，通过 MTT assay 检测细胞活力。 结果: 处理 24 h 后，Genistein 对浓度超过 80 µM 的 MCF-7 细胞具有细胞毒性。用 Genistein 50 µM 以上处理 48 h，在 MCF-7 细胞中诱导细胞毒性。[1] 方法: MCF-7 细胞或分化中的 3T3-L1 细胞用 Genistein (50-200 µM) 处理 24-72 h，通过 Western Blot 检测靶点蛋白表达水平。 结果: MCF-7 细胞中，所有浓度的 Genistein 均下调 ERα 的表达；此外，Genistein 在 48 和 72 h 后的作用更大。与阴性对照 (3T3-L1 前脂肪细胞) 相比，Genistein 处理 48 h 后，3T3-L1 细胞中 ERα 的表达上调了 1.98 倍，以启动分化。[2] |
| 体内活性 | 方法: 为检测体内抗肿瘤活性，将 Genistein (2-20 mg/kg in 25 mmol/L Na2CO3) 灌胃给药给携带 B16F10 肿瘤的 B6C3F1 小鼠，每天一次，持续 28 天。 结果: 与 Genistein 的化学预防作用一致，暴露于该化合物显著增加了宿主对 B16F10 肿瘤的抵抗力，这反映在中高剂量水平肿瘤细胞注射后肺肿瘤结节数量的减少上。[3] |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 5.4 mg/mL (19.98 mM), Solution. DMSO : 262.5 mg/mL (971.36 mM), Sonication is recommended. H2O : < 1 mg/mL (insoluble or slightly soluble) Ethanol : 2 mg/mL (7.4 mM), Sonication is recommended. |
| 关键字 | Inhibitor | inhibit | HER1 | Genistein | ErbB-1 | Epidermal growth factor receptor | EndogenousMetabolite | Endogenous Metabolite | EGFR | Autophagy | Apoptosis |
| 相关产品 | Guanidine hydrochloride | Glycerol | Aceglutamide | Malic acid | DL-Lysine | Sucrose | Hemin | Nicotinamide riboside malate | Gluconate Calcium | Thymidine | Corn starch | D(+)-Raffinose pentahydrate |
| 相关库 | Inhibitor Library | Anti-Cancer Active Compound Library | Bioactive Compounds Library Max | Selected Plant-Sourced Compound Library | Miao medicine Compound Library | Kinase Inhibitor Library | Bitter Compound library | Immunology/Inflammation Compound Library | Membrane Protein-targeted Compound Library | Tyrosine Kinase Inhibitor Library | Anti-Inflammatory Traditional Chinese Medicine Compound Library | Polyphenolic Natural Product Library |
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23,974 29.041 24 NATURE MEDICINE 60,002 28.054 25 PROGRESS IN POLYMER SCIENCE 17,446 26.854 26 Annual Review
110 137 195 1:2 1097 239 134 100 84 75 71 68 72 82 103
480.12～538.08 60 513.5 29.18 492.62～534.38 61 476.4 42.06 446.32～506.48 62 511.5 28.46 491.14～531.86 63 480.7 44.83
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￥198 - 591
