- ￥2600
- Proteintech
- 15.6-1000 pg/mL
- KE10045
- 2026年07月08日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 检测范围：
15.6-1000 pg/mL
- 应用：
Sandwich ELISA
- 适应物种：
Mouse
- 样本：
Serum, Plasma, Cell culture supernates, Urine
- 规格：
96T
Product Information
KE10045 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse NGAL ELISA kit is to be used to detect and quantify protein levels of endogenous mouse NGAL. The assay recognizes mouse NGAL. An antibody specific for mouse NGAL has been pre-coated onto the microwells. The mouse NGAL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse NGAL is added to detect the captured mouse NGAL protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse NGAL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 4.0 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16819 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 106% | 95%-120% |
| Mouse urines | 116% | 92%-131% |
| Cell culture supernates | 106% | 79%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 429.5 | 33.2 | 7.7 |
| 2 | 20 | 109.1 | 4.7 | 4.3 |
| 3 | 20 | 26.6 | 2.0 | 7.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 486.3 | 40.4 | 8.3 |
| 2 | 24 | 114.7 | 6.3 | 5.5 |
| 3 | 24 | 20.5 | 1.7 | 8.5 |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL; also known as neutrophil lipocalin, lipocalin-2, siderocalin, 24p3, or LCN2) is a small molecule of almost 25 kd that belongs to the well-defined superfamily of proteins called lipocalins. NGAL initially found in activated neutrophils, in accordance with its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Lcn2, Lipocalin-2, Lipocalin 2, Neutrophil gelatinase-associated lipocalin, Oncogene 24p3 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
人Lipocalin-2 (NGAL)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 Lipocalin-2 ( NGAL )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液、唾液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 NGAL 单抗包被于酶标板上，标准品和样品中的 NGAL与单抗结合，加入生物素化的抗人
licanming 请问有无人用过assaydesigns公司的PKC Kinase Activity Assay Kit（cat NO:EKS-420A）?可否说一下应用的经验？注意事项等？还有，该试剂盒的说明书有几处地方不是很懂，请大家帮忙看一下： 1、To avoid cross contamination, change disposable pipette tips between the addition of each standard
技术资料暂无技术资料 索取技术资料
