- ￥2600
- Proteintech
- 93.8-6000 pg/mL
- KE00147
- 2026年07月07日
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- 文献和实验
- 技术资料
- 检测范围：
93.8-6000 pg/mL
- 应用：
Sandwich ELISA
- 适应物种：
Human
- 样本：
Cell lysates
- 规格：
96T
Product Information
KE00147 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ATF4 ELISA kit is to be used to detect and quantify protein levels of endogenous ATF4. The assay recognizes human ATF4. An antibody specific for ATF4 has been pre-coated onto the microwells. The ATF4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for ATF4 is added to detect the captured ATF4 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ATF4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 77.8 pg/mL |
| Range | 93.8-6000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 468 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 88% | 70%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 279.9 | 18.0 | 6.4 |
| 2 | 20 | 878.4 | 44.9 | 5.1 |
| 3 | 20 | 3,600.8 | 170.7 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 290.2 | 24.1 | 8.3 |
| 2 | 24 | 883.5 | 56.3 | 6.4 |
| 3 | 24 | 3,553.9 | 139.6 | 3.9 |
Background Information
ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CREB 2, CREB2, DNA binding protein TAXREB67, TAXREB67, TXREB |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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文献和实验licanming 请问有无人用过assaydesigns公司的PKC Kinase Activity Assay Kit（cat NO:EKS-420A）?可否说一下应用的经验？注意事项等？还有，该试剂盒的说明书有几处地方不是很懂，请大家帮忙看一下： 1、To avoid cross contamination, change disposable pipette tips between the addition of each standard
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
R&D Systems Quantikine® 人高分子量脂联素ELISA试剂盒
浆中HMW 脂联素的水平，而不需要对样品进行蛋白水解的预处理。关于脂联素相关产品的更多信息，请访问我们的网站www.RnDSystems.com/Adipocytokines。人HMW 脂联素水平的测定。利用Quantikine Human HMW Adiponectin/Acrp30ELISA Kit（货号 DHWAD0），我们分析了来自不同BMI 个体的血清中的HMW 脂联素平均水平，包括低BMI（平均BMI = 19.8 ；N=4），高BMI（平均BMI = 32.8 ；N=4）和超高BMI（平均
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