- ￥2800
- Proteintech
- 31.25-2000 pg/mL (PT 4-ef); 62.5-4000 pg/mL (PT 3)
- SE50257
- 2026年11月05日
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- 文献和实验
- 技术资料
- 检测范围：
31.25-2000 pg/mL (PT 4-ef); 62.5-4000 pg/mL (PT 3)
- 应用：
Sandwich ELISA
- 适应物种：
Human
- 样本：
Serum, Plasma, Cell culture supernatant
- 规格：
96T
Product Information
| Product name | Speedy™ Human CXCL6 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.6 pg/mL |
| Range | 31.25-2000 pg/mL ; 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6372 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 100% | 93%-104% |
| Cell culture supernatant | 85% | 76%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,020.3 | 20.8 | 2.0 |
| 2 | 8 | 237.8 | 5.9 | 2.5 |
| 3 | 8 | 121.3 | 2.8 | 2.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,026.3 | 27.9 | 2.7 |
| 2 | 16 | 238.3 | 6.2 | 2.6 |
| 3 | 16 | 120.7 | 2.9 | 2.4 |
Background Information
Chemokine (C-X-C motif) ligand 6 (CXCL6) is a small cytokine belonging to the CXC chemokine family that is also known as granulocyte chemotactic protein 2 (GCP-2). As its former name suggests, CXCL6 is a chemoattractant for neutrophilic granulocytes. It elicits its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2.The gene for CXCL6 is located on human chromosome 4 in a cluster with other CXC chemokine genes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GCP-2, C X C motif chemokine 6, CXCL 6, CXCL-6 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
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