- ￥2600
- Proteintech
- 0.2-12.5 ng/mL
- KE30008
- 2026年07月06日
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- 文献和实验
- 技术资料
- 检测范围：
0.2-12.5 ng/mL
- 应用：
Sandwich ELISA
- 适应物种：
Bacteriophage T7
- 样本：
T7 RNA polymerase
- 规格：
96T
Product Information
KE30008 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The bacteriophage T7 RNA polymerase ELISA kit is to be used to detect and quantify protein levels of endogenous bacteriophage T7 RNA polymerase. The assay recognizes bacteriophage T7 RNA polymerase. An antibody specific for bacteriophage T7 RNA polymerase has been pre-coated onto the microwells. The bacteriophage T7 RNA polymerase protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for bacteriophage T7 RNA polymerase is added to detect the captured bacteriophage T7 RNA polymerase protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Bacteriophage T7 RNA polymerase ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | T7 RNA polymerase 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.2-12.5 ng/mL |
| Reactivity | Bacteriophage T7 |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1261050 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| T7 RNA polymerase | 93% | 91%-94% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 7.18 | 0.15 | 2.14 |
| 2 | 8 | 1.73 | 0.06 | 3.47 |
| 3 | 8 | 0.84 | 0.02 | 2.60 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 7.06 | 0.28 | 4.03 |
| 2 | 16 | 1.74 | 0.08 | 4.48 |
| 3 | 16 | 0.85 | 0.06 | 6.59 |
Background Information
T7 RNA polymerase is a DNA-dependent RNA polymerase from bacteriophage T7, known for its high specificity and processivity. T7 RNA polymerase requires DNA templates and magnesium ions (Mg2+) as cofactors together in the process of RNA synthesis. Bovine serum proteins and spermine have a promoting effect on T7 RNA polymerase. One of its differences from bacterial RNA polymerase is that T7 RNA polymerase is not inhibited by the antibiotic rifamycin. T7 RNA polymerase is widely used in biotechnology for mRNA synthesis, ribozyme production, and synthetic biology applications. Its single-subunit structure (~99 kDa) simplifies engineering for improved properties. Recent studies highlight its role in CRISPR-based diagnostics due to rapid RNA amplification.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | T7 polymerase, T7 RNA polymerase |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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文献和实验看说明书上是写着用ＣsＣl梯度离心的方式，除此外还有别的吗？最好是所涉及的器材可方便取得的。还有啊，像M13之类的噬菌体可以用PEG/NaCl方法得到其DNA，这个T7就不适合了？多谢各位支持。 相关的例子: 1.取活鲤鱼肝胰脏,经冲洗后,立即投入液氮中.然后制成匀浆.先在4℃下600g离心,保留上层液.再900g离心,保留沉淀物并用不同溶液重复悬浮洗涤离心,可得到比较纯的线粒体.将上述线粒体在含1%SDS的Saline-Na_2EDTA溶液中悬浮,于37℃反应15min.然后在室温
mRNA Amplification with T7 RNA Polymerase
on a 42ºC oven and set PCR machine to hold at 70ºC. In an Eppendorf tube add: 1 µg Total RNA 1 µL T7 oligo(dT) primer q.s. to 12 µL with Nuclease-free H2 O Incubate 10 min. at 70ºC. Spin briefly to pull
亦称 RNA 型噬菌体。是遗传物质为 RNA 的噬菌体之总称。自从 1961 年洛布和津德（ T.Loeb 和 N.D.Zinder ）发现 f 2 噬菌体以来，巳经分离出许多噬菌体，而且均以大肠杆菌的雄株为害主。如 f 2 、 MS 2 、 R 17 、 Q β、 fr 等。根据血清学的亲缘性和其它生物学性质、物理化学的性质而分为几群。都是直径约 20nm 的球状对称结构，在颗粒中含有一条单链的 RNA 。 RNA 的分子量约为 1.1 × 10
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