- ￥2600
- Proteintech
- 62.5-4000 pg/mL
- KE00560
- 2026年07月06日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
从原料到标记，打造属于中国自己的流式抗体品牌Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 检测范围：
62.5-4000 pg/mL
- 应用：
Sandwich ELISA
- 适应物种：
Human
- 样本：
Serum, Plasma, Cell culture supernatant, Urine, Human milk, Cell lysate, Tissue lysate
- 规格：
96T
Product Information
KE00560 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human HER2/ErbB2 ELISA kit is to be used to detect and quantify protein levels of endogenous human HER2/ErbB2. The assay recognizes human HER2/ErbB2. An antibody specific for human HER2/ErbB2 has been pre-coated onto the microwells. The human HER2/ErbB2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human HER2/ErbB2 is added to detect the captured human HER2/ErbB2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HER2/ErbB2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Human milk, Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2064 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human milk | 103% | 101%-107% |
| Lysate | 108% | 102%-117% |
| Urine | 97% | 93%-102% |
| Cell culture supernatant | 103% | 99%-110% |
| Human serum | 101% | 96%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 603.2 | 25.3 | 4.2 |
| 2 | 8 | 152.9 | 6.8 | 4.5 |
| 3 | 8 | 65.0 | 4.5 | 6.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 985.3 | 25.6 | 2.6 |
| 2 | 16 | 248.7 | 7.6 | 3.0 |
| 3 | 16 | 124.5 | 5.7 | 4.6 |
Background Information
The human epidermal growth factor receptor (HER) family of receptors plays a central role in the pathogenesis of several human cancers. HER2 (human epidermal growth factor receptor 2), also known as ErbB2 and Neu, is 1 of the 4 membrane receptor tyrosine kinases (RTKs) and when activated affects cell proliferation and survival. HER2 is expressed in many tissues and its major role in these tissues is to facilitate excessive/uncontrolled cell growth and tumorigenesis. Additionally, HER2 mutations have also been reported in common cancers such as pulmonary, colorectal, and breast cancers, indicating a potential for HER2-directed therapies in these cancers.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ERBB2, CD340, HER 2, HER 2/neu, HER2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验：分别是竞争性蛋白质结合分析法[3]、放射性免疫分析法[4]和薄层色谱法[5]。在80年代，放射性免疫分析法得到了广泛的应用和改进，由于这种方法采用放射性核素标记，应用范围受到一定限制，为了进一步提高检测的灵敏度和操作的安全性，进入90年代又陆续发明了各种酶标记法和化学发光法的竞争性ELISA检测试剂盒，这一类检测方法的根本原理都是基于抗体抗原的免疫反应，所有这些试剂盒中采用的抗体全部是兔抗血清或者是经过特异亲和提纯之后的抗cAMP或cGMP的兔多抗，毫无疑问，兔多抗在约四十年的cAMP，cGMP定量
发光检测仪 ELISA检测试剂盒 ELISPOT检测 ELISA试剂盒 抗体 酶标板
特异性。需要用不同的抗原和酶联物来检测相应的抗体，不可相互“通用” 。因此试剂生产成本较高。 其他应用： · ELISA：采用双抗夹心ELISA检测HSV1。 · 免疫胶体金：利用免疫层析原理制备免疫胶体金试纸条来检测HSV1。 · 免疫荧光：采用自制荧光标记HSV1 McAb以直接免疫荧光法(DFA)检测HSV1，以期可获得一种用于临床应用的快速检测HSV抗原的方法。 八、四正柏生物简介 北京四正柏生物科技有限公司（Beijing
技术资料暂无技术资料 索取技术资料
