- ￥2600
- Proteintech
- 125-8000 pg/mL
- KE00442
- 2026年07月06日
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- 文献和实验
- 技术资料
- 检测范围：
125-8000 pg/mL
- 应用：
Sandwich ELISA
- 适应物种：
Human
- 样本：
Cell lysate
- 规格：
96T
Product Information
KE00442 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human HIF2α/EPAS1 ELISA kit is to be used to detect and quantify protein levels of endogenous human HIF2α/EPAS1. The assay recognizes human HIF2α/EPAS1. An antibody specific for human HIF2α/EPAS1 has been pre-coated onto the microwells. The human HIF2α/EPAS1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human HIF2α/EPAS1 is added to detect the captured human HIF2α/EPAS1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HIF2α/EPAS1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 45.7 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2034 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 86% | 79%-94% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 3,871.0 | 132.7 | 3.4 |
| 2 | 8 | 1,139.1 | 31.0 | 2.7 |
| 3 | 8 | 537.0 | 30.3 | 5.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 3,729.4 | 243.9 | 6.5 |
| 2 | 16 | 1,090.7 | 60.2 | 5.5 |
| 3 | 16 | 543.0 | 34.4 | 6.3 |
Background Information
HIF2A, also named as EPAS1, is a 870 amino acid protein, which is expressed in most tissues, with highest levels in placenta, lung and heart. HIF2A colocalizes with HIF3A in the nucleus and speckles. HIF2A as a transcription factor involves in the induction of oxygen regulated genes. HIF2A binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. HIF2A regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. HIF2A may also play a role in the formation of the endothelium that gives rise to the blood brain barrier. The calculated molecular weight of HIF2A is 96 kDa, but In normoxia, HIF2A is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EPAS1,Basic-helix-loop-helix-PAS protein MOP2,bHLH |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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文献和实验竞争ELISA检测试剂盒，无需对样品中的cAMP进行乙酰化。 http://www.neweastbio.com/NonAcetylatedCamp.do 小猪亨亨 http://www.mediomics.com/camp_desinger.html 我准备试试这个 neweastbio 如何定量检测cAMP和cGMP
组成，其结构的不同处在β亚单位，而两者的α亚单位是同类的。用hCG免疫动物所得的抗血清中含有抗α-hCG和抗β-hCG两种抗体，抗α-hCG抗体将与LH发生交叉反应。在临床检验中，如用抗hCG抗血清作为妊娠诊断试剂检定尿液中hCG，只能用于hCG浓度较高的试验，否则妇女生理性排泄入尿液中的微量LH将与之发生交叉反应。因此在作为早孕诊断（敏感度应达到50mIu/mlhCG）的实际中必须应用只对hCG特异的抗β-hCG，以避免与其它激素的交叉反应的发生。1.3.4 敏感性 在测定血清中某一物质的含量时
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