- ￥2600
- Proteintech
- 7.8-500 pg/mL
- KE00385
- 2026年07月06日
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- 文献和实验
- 技术资料
- 检测范围：
7.8-500 pg/mL
- 应用：
Sandwich ELISA
- 适应物种：
Human
- 样本：
Serum, Plasma, Cell culture supernatant
- 规格：
96T
Product Information
KE00385 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IL-6 ELISA kit is to be used to detect and quantify protein levels of endogenous human IL-6. The assay recognizes human IL-6. An antibody specific for human IL-6 has been pre-coated onto the microwells. The human IL-6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IL-6 is added to detect the captured human IL-6 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-6 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3569 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 90% | 86%-99% |
| Human serum | 82% | 72%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 229.9 | 9.0 | 3.9 |
| 2 | 20 | 52.2 | 1.7 | 3.3 |
| 3 | 20 | 15.2 | 0.8 | 5.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 242.1 | 11.3 | 4.6 |
| 2 | 24 | 55.5 | 1.8 | 3.2 |
| 3 | 24 | 15.7 | 0.9 | 5.5 |
Background Information
Interleukin-6 (IL6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL6,IL-6,B-cell stimulatory factor 2,IFN-beta-2,IL |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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文献和实验葡萄糖来源 AGEs ELISA 检测试剂盒 （ Glucose-derived AGEs ELISA Kit ） 研 究 背 景 越来越多的证据指出晚期糖基化终端产物（ AGEs ）修饰蛋白严重影响到老龄化人群的健康问题。 AGEs 是葡萄糖与自由的蛋白质氨基基团发生非酶促糖基化反应的终端不可逆代谢产物的总称。其在人体内的积累会引发组织或器官一系列的病理性变化，从而导致白内障、阿尔茨海默氏病、骨关节炎、心功
紊乱，且存在一定程度增生和胞内囊泡结构。 3、类器官活死染 活死染显示：与Control相比，Model类器官活性有所减弱。 4、FITC葡聚糖荧光染色 FITC葡聚糖荧光染色：与Control相比，Model类器官通透性增强。 5、IL-6和TNF-α检测 IL-6和TNF-α检测显示：与Control相比，Model类器官通透性增强，Modle组ELISA检测IL-6显著升高，图 (A)；Modle组ELISA检测TNF-α显著升高，图 (B)。 总结：通过对Control（非炎症诱导组
特异性。需要用不同的抗原和酶联物来检测相应的抗体，不可相互“通用” 。因此试剂生产成本较高。 其他应用： · ELISA：采用双抗夹心ELISA检测HSV1。 · 免疫胶体金：利用免疫层析原理制备免疫胶体金试纸条来检测HSV1。 · 免疫荧光：采用自制荧光标记HSV1 McAb以直接免疫荧光法(DFA)检测HSV1，以期可获得一种用于临床应用的快速检测HSV抗原的方法。 八、四正柏生物简介 北京四正柏生物科技有限公司（Beijing
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