- ￥2600
- Proteintech
- 78.1-5000 pg/mL
- KE00616
- 2026年07月06日
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- 文献和实验
- 技术资料
- 检测范围：
78.1-5000 pg/mL
- 应用：
Sandwich ELISA
- 适应物种：
Human
- 样本：
Cell lysate
- 规格：
96T
Product Information
KE00616 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MRP3/ABCC3 ELISA kit is to be used to detect and quantify protein levels of endogenous human MRP3/ABCC3. The assay recognizes human MRP3/ABCC3. An antibody specific for human MRP3/ABCC3 has been pre-coated onto the microwells. The human MRP3/ABCC3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MRP3/ABCC3 is added to detect the captured human MRP3/ABCC3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MRP3/ABCC3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 13.8 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8714 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 83% | 76%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,162.1 | 112.2 | 5.2 |
| 2 | 8 | 554.9 | 19.5 | 3.5 |
| 3 | 8 | 288.6 | 10.2 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,032.0 | 183.3 | 9.0 |
| 2 | 16 | 513.9 | 54.3 | 10.6 |
| 3 | 16 | 272.6 | 26.3 | 9.6 |
Background Information
MRP3 also known as ABCC3, belongs to ATP-binding cassette (ABC) transporter protein superfamily. MRP3 hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants, and endogenous compounds across cell membranes. In normal liver, MRP3 was found present mainly in the bile ducts. In livers of patients with various forms of cholestasis, MRP3 levels were frequently increased in the proliferative cholangiocytes, suggesting its role in cholehepatic and enterohepatic bile circulation and in protecting liver from toxic bile salts.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ABCC3, MRP3, ABC31, ATP-binding cassette sub-family C member 3, EC:7.6.2.- |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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文献和实验：分别是竞争性蛋白质结合分析法[3]、放射性免疫分析法[4]和薄层色谱法[5]。在80年代，放射性免疫分析法得到了广泛的应用和改进，由于这种方法采用放射性核素标记，应用范围受到一定限制，为了进一步提高检测的灵敏度和操作的安全性，进入90年代又陆续发明了各种酶标记法和化学发光法的竞争性ELISA检测试剂盒，这一类检测方法的根本原理都是基于抗体抗原的免疫反应，所有这些试剂盒中采用的抗体全部是兔抗血清或者是经过特异亲和提纯之后的抗cAMP或cGMP的兔多抗，毫无疑问，兔多抗在约四十年的cAMP，cGMP定量
发光检测仪 ELISA检测试剂盒 ELISPOT检测 ELISA试剂盒 抗体 酶标板
特异性。需要用不同的抗原和酶联物来检测相应的抗体，不可相互“通用” 。因此试剂生产成本较高。 其他应用： · ELISA：采用双抗夹心ELISA检测HSV1。 · 免疫胶体金：利用免疫层析原理制备免疫胶体金试纸条来检测HSV1。 · 免疫荧光：采用自制荧光标记HSV1 McAb以直接免疫荧光法(DFA)检测HSV1，以期可获得一种用于临床应用的快速检测HSV抗原的方法。 八、四正柏生物简介 北京四正柏生物科技有限公司（Beijing
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