- ￥10480 - 18880
- Sino Biological
- 北京
- 30115-H94H-NA-B
- 2026年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human LGR5 Protein (Nanodisc, Flag & His & AVI Tag), Biotinylated
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10480.00/18880.00
| 规格： | 10480.00 | 产品价格： | ￥10480.0 |
|---|---|---|---|
| 规格： | 18880.00 | 产品价格： | ￥18880.0 |
蛋白名称：重组人LGR5蛋白(Nanodisc, Flag & His & AVI 标签), 生物素标记
蛋白构建：A DNA sequence encoding the Human LGR5 (O75473) (Gly22-Ser844) was expressed, with a flag tag at the N-terminus,and a C-terminal polyhistidine tag followed by an AVI tag.The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. This protein was prepared using our Nanodisc platform. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
表达宿主：HEK293 Cells≥ 80 % as determined by SDS-PAGE.
蛋白活性：1. Immobilized Recombinant Human LGR5 Protein (Nanodisc, Flag & His & AVI Tag), Biotinylated (Cat: 30115-H94H-NA-B) at 1 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Petosemtamab Derived Anti- LGR5 Monoclonal antibody, Human IgG1, the EC50 is 0.5-3 ng/mL (QC tested). 2. Immobilized Petosemtamab Derived Anti- LGR5 Monoclonal antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human LGR5 Protein (Nanodisc, Flag & His & AVI Tag), Biotinylated (Cat: 30115-H94H-NA-B), the EC50 is 10.6 ng/mL (Routinely tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Asp
蛋白分子量：The recombinant Human LGR5 comprises 866 amino acids and has a predicted molecular mass of 95.95 kDa. As a result of glycosylation, the band migrates approximately 92 kDa in the SDS-PAGE reducing conditions.
蛋白NP号：O75473
蛋白氨基酸序列：Gly22-Ser844
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文献和实验T1.1 =26.2 T1.2 =43-26.2=16.8 T2.1 =53-26.2=26.8 T2.2 =44-26.2=17.2 3.计算x2 值 按公式20.12代入 4．查x2 值表求P值 在查表之前应知本题自由度。按x2 检验的自由度v=（行数-1）（列数-1），则该题的自由度v=（2-1）（2-1）=1，查x2 界值表（附表20-1），找到x2 0.001
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 26S 蛋白酶体 (26S PSM)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液、唾液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 26S PSM 单抗包被于酶标板上，标准品和样品中的 26S PSM 与单抗结合，加入生物素化的抗人 26S PSM
。 9. 最后，样品加入叠氮钠（终浓度0.5 g/L）及1.0 g/L BSA。将结合产物置4℃，避光保存，亦可加入50%重蒸甘油，置-20℃保存。 要点： 1. 如在反应混合液中有叠氮钠或游离氨基存在，会抑制标记反应。因此，蛋白质在反应前要对0.1 mol/L 碳酸氢钠缓冲液或0.5 mol/L 硼酸缓冲液 2. 所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同，选择不当则影响标记的效率，应先用几个不同的分子比来筛选最适条件。
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