RNA Clean & Concentrator™-5 (5

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™

E.Z.N.A.® Mag-Bind® Viral DNA/RNA Spin Protocol

实验原理   If using the E.Z.N.A.™ Mag-Bind® Viral DNA/RNA Kit for the first time, please read this booklet to become familiar with the procedure and its various modification. Samples are lysed in a specially formulated buffer

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications

Lipofectamine 2000™ (Invitrogen) at a 1:3 ratio (e.g. 1µl plasmid to 3µl lipofectamine). The plasmid and lipofectamine are mixed together, vortexed, pulse spun, and then 50µl of DMEM (without Fetal bovine serum, FBS) added. The entire mixture is again vortexed