Quick-DNA Fecal/Soil Microbe M

Midiprep preparation of Plasmid DNA

实验概要 The PureLink™ HiPure Plasmid DNA Midiprep Kit allows purification of 100–350 μg of high-quality plasmid DNA from 15–25 mL overnight E. coli cultures in ~2 hours when cloning high copy number plasmids

MicroElute DNA Clean-Up Kit

. Proceed step 2. Tips: 1 volume of Buffer P3 and 2 volume of absolute ethanol can be premixed. Then add 1.5 x volume of this mixture into DNA solution. Purify DNA by HiBindTM MicroEluteTM DNA Column    2) Place a MicroElute HiBind™ DNA

Mag-Bind Soil DNA Kit Protoco

1. Weigh 500 mg of glass beads in a 2 ml centrifuge tube, add 0.2-0.5 g soil sample. Add 0.8 ml Buffer SLX Mlus. Vortex at maximum speed for 3-5 minute to lyse samples. For the best result, A Mixer Mill, such as Fastprep-24, Mixer Mill MM 300