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- GenScript
- A01857
- 2026年06月29日
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- 规格：
40 μg
| Specificity | GenScript MonoRab™ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit specifically recognizes His tags placed at C-terminal and internal regions of His-fusion proteins. This product is not recommended for N-terminal His-fusion protein detection. |
| Host Species | Rabbit |
| Immunogen | 6x His synthetic peptide coupled to KLH |
| Conjugate | Unconjugated |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||||||||||
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| Form | Lyophilized |
| Storage Buffer | Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL. |
| Storage Instructions | The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C, or for 12 months at -20℃ or below. Upon receiving, spin the vial down to place the lyophilized antibody on the bottom of the vial. Reconstitute the antibody as mentioned above. Prepare single use aliquots (no less than 10µl) and freeze it immediately at -20℃. The frozen aliquots should be thawed once with any remainder kept at 4℃. |
| Purification | Purified by Protein A affinity chromatograph. |
| Isotype | Rabbit IgG |
| Clonality | Monoclonal |
| Clone ID | 25B6E11 |
| Note | GenScript can customize this product according to you requirement, including product size, buffer components, etc. |
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文献和实验祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
货号T501，产品描述：HA-Tag Rabbit Polyclonal Antibody；规格 50 ul / 100 ul； 货号T503，产品描述：Anti-DYKDDDDK Polyclonal Antibody；规格 50 ul / 100 ul； 货号T504，产品描述：Myc-Tag Mouse Monoclonal Antibody；规格 50 ul / 100 ul； 货号
(against the third target protein) followed by detection using a mixture of Streptavidin NorthernLights™ NL-493 tag (green fluorescence) and anti-DIG secondary antibody conjugated to a Rhodamine Red X™ tag (red fluorescence). This technique provides good spectral separation
antibody made in goat (Vector Labs): 1:550 (i.e. 0.9µl in 500µl blocking solution) Rhodamine-anti-Digoxigenin antibody made in sheep (Roche): 1:45 (i.e. 11µl in 500µl blocking solution) DAPI (4'6' Diamidino-2-phenylindole) stock solution
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