- ￥1500
- GenScript
- A01796
- 2026年06月29日
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- 文献和实验
- 技术资料
- 规格：
100μg
| Specificity | GenScript THE™ PEG Antibody [Biotin], mAb, Mouse detects PEG backbone. The product can detect the variety of PEGs such as PEG40K, PEG20K, PEG5K, PEG12, PEGylated drugs and PEG conjugates. |
| Host Species | Mouse |
| Immunogen | PEG conjugated to KLH |
| Conjugate | Biotin |
| Working concentrations for specific applications should be empirically determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the detection methods, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
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| Form | Lyophilized |
| Storage Buffer | lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. For long-term storage, adding glycerol to a final concentration of 50% (v/v) in the reconstituted antibody solution is recommended. |
| Storage Instructions | The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles. |
| Purification | Affinity chromatography |
| Isotype | Mouse IgM |
| Clonality | Monoclonal |
| Clone ID | 5E10E9 |
| Note | Some reagents such as Tween-20, Triton X-100 and NP-40 interfere with the PEG antibody interaction due to structural similarity to PEG. Antibody solution should not contain such reagents in the assay of PEGylated molecules. |
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文献和实验1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight.(2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00)(3) 50 mM Tris pH7.8.(4) 40 g ammonium sulfate for every 100 ml Sup.(5) 4L 100 mM Tris/pH
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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