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- 文献和实验
- 技术资料
- 供应商：
AssayPro
- 标记物：
IgG
- 克隆性：
多克隆性抗体
- 规格：
150 ug
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文献和实验Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation&
with minimal loss of surface antigen expression and antibody-antigen association. For some cell types, the presence of apoptotic cells based on reduced DNA content can also be detected. One such technique employs the addition of ethanol to cells previously
Simultaneous analysis of DNA content and surface
content with simultaneous preservation of cell surface markers, we have utilized gentle ethanol treatment techniques, which permeablize cells with minimal loss of surface antigen expression and antibody-antigen association. For some cell types
Silver Enhancement of Colloidal Gold
. for 1-nm gold, for Nanogold 4 to 5 min (20 - 22°C). The final pH is 4 (see Burry 1995, p. 220). Remove blocking buffer, add 25 µl of the primary antibody solution per coverslip (with a final concentration in the range of 1-5 µg specific IgG/ml) and incubate
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