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- 文献和实验
- 技术资料
- 供应商:
AssayPro
- 标记物:
IgG-RPE Conjugate
- 克隆性:
多克隆性抗体
- 规格:
5 x 30 ug
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v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
(7) . GSK3 is one of the most unusual kinases of the 500 or soencoded in the human genome. Firstly, most (if not all) substratesrequire prior phosphorylation by another kinase beforethey can be efficiently phosphorylated by GSK3. This process isknown
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primary antibody solution, and wash membrane 4×4 min with TTBS at room temperature. 11. After the last wash, incubate the membrane in solution containingHRP–secondary antibody conjugate (donkey anti-goatIgG-HRP at a 1:1,000 dilution) for 1 h at room
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