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东莞汉诺
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1 plate
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文献和实验Dynamic Flow Assay in a Parallel Plate Flow Chamber
of 10�/ml to coating region. Incubate 1 hr. 3. Aspirate off liquid, add 20� of 1% BSA to block for 1 hr. 4. Aspirate off BSA, add 20� of inhibitor for 1 hr. 5. Dishes are ready for use in flow assay. Flow Assay Using Parallel Plate Flow
of 7 x 2 x 0.5" and bottom dimensions of 7 x 2 x 0.25". 2) Recess the top plate in the center portion to 0.125" deep, leaving 0.950" per end for 1.375" wide x 0.3125" deep fluid reservoir. 3) Drill 1/16" wide x 0.625" long
Coating of Platelets with Antibody in vitro
or an Ab directed to platelet Ag (you may use Grener F-bottom microtitration plate for this step). If the Ab used is rat IgG1 (k) against mouse CD41 (MWReg30, Becton Dickinson, Cat.: 09911D) then the advised concentration is 0.6µg/ml (0.15µg per 200µl
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