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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
It can be stored for 12 months at temperatures ranging from -20°C to -80°C. It is recommended to allocate optimal storage conditions for the protein. Avoid repeated freeze-thaw cycles.
- 保质期:
12个月
- 英文名:
1110029L17Rik;2310051F07Rik;AI225898;AL022874;AW546247;Eif2c2;ENSMUSG00000072493;Gerp95;Gm10365;mKIAA4215
- 库存:
99
- 供应商:
湖北安可诺生物工程有限公司
- 规格:
20ug
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文献和实验Protein Components of the microRNA Pathway and Human Diseases
and function, such as the ribonucleases III (RNases III) Drosha and Dicer as well as Argonaute 2 (Ago2), appear to be essential. However, the accessory proteins of the miRNA pathway, such as the DiGeorge syndrome critical region gene 8 (DGCR8) protein
沉淀法里的应用 RNA免疫沉淀试剂人Ago2里,使用本方法的二氧化硅磁珠里的固相化了抗Argonaute抗体的磁珠,从3种人培养细胞株(HeLa、HepG2、HEK293)及小鼠培养细胞株(P388D1)中纯化结合到Ago2的microRNA。纯化样品进行UREA-PAGE后,根据银染的结果,从人培养细胞株中高纯度纯化结合到Ago2的约22个碱基的microRNA(因为抗体不反应因此不能纯化小鼠细胞株)。使用的细胞数大约为5 X 106 cells 固相化抗体外的蛋白质 最后再介绍
Identification of Components of RNAi Pathways Using the Tandem Affinity Purification Method
affinity purification (TAP) method (18 –20 ) to isolate protein components of RISC in cultured Drosophila Schneider-2 (S2) cells. This purification method has allowed us to identify several RISC components, including Argonaute 2 (AGO2), the Drosophila
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