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- 安可诺
- 湖北武汉
- AK03030
- 2026年06月21日
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- 文献和实验
- 技术资料
- 保存条件：
It can be stored for 12 months at temperatures ranging from -20°C to -80°C. It is recommended to allocate optimal storage conditions for the protein. Avoid repeated freeze-thaw cycles.
- 保质期：
12个月
- 英文名：
Uracil-DNA-glycosylase, ECK2578, JW2564
- 库存：
99
- 供应商：
湖北安可诺生物工程有限公司
- 规格：
20ug
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文献和实验Expression of a cDNA in E.coli in order to prepare an antiserum
an antiserum. As an alternative, the cDNA can be expressed in E.coli as a translational fusion and the expressed protein used to prepare the antiserum. This is normally done as fusion constructs with e.g. -galactosidase ( LacZ
Analysis of total E.coli protein by SDS PAGE
Hahn Lab 1.In microfuge tubes,spin down 0.1 ml of uninduced cells grown to near saturation or 0.15 ml of IPTG induced cells.Remove YT (or LB)media with a pipetman or drawn out pasteur pipette and freeze cell pellets for later analysis. 2.Add 120
Generation of Recombinant Baculovirus DNA in E.coli Using a Baculovirus Shuttle Vector
of the Autographa californica nuclear polyhedrosis virus (AcNPV) are abundantly expressed during the late stages of infection. The recombinant proteins are usually soluble and functionally similar to their authentic counterparts (l –7 ). In the following sections
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