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- 文献和实验
- 技术资料
- 保存条件:
It can be stored for 12 months at temperatures ranging from -20°C to -80°C. It is recommended to allocate optimal storage conditions for the protein. Avoid repeated freeze-thaw cycles.
- 保质期:
12个月
- 英文名:
DNA Fragmentation Factor Subunit Alpha; DNA Fragmentation Factor 45 kDa Subunit; DFF-45; Inhibitor of CAD; ICAD; DFFA; DFF1; DFF45; H13
- 库存:
99
- 供应商:
湖北安可诺生物工程有限公司
- 规格:
20ug
产品概述
重组人 DNA 片段化因子亚基 α/DFFA/DFF45/ICAD 蛋白,由大肠杆菌表达,带有N 端 His 标签(N-His)。
产品参数
| 英文名称 | Recombinant Human DNA Fragmentation Factor Subunit α/DFFA/DFF45/ICAD Protein |
| 物种来源 | 人 |
| 表达宿主 | 大肠杆菌 |
| 表达区段 | 第 1–331 位氨基酸(aa) |
| 纯度 | 90% |
| 分子量 | 38.7 kDa |
| 标签 | N 端 His 标签(N-His) |
| 缓冲液 | 磷酸盐缓冲盐水(PBS) |
| 内毒素 | <1.0EU/μg |
| UniProt 编号 | O00273 |
| 别名(标准名称/缩写) | DNA Fragmentation Factor Subunit Alpha; DNA Fragmentation Factor 45 kDa Subunit; DFF-45; Inhibitor of CAD; ICAD; DFFA; DFF1; DFF45; H13 |
复溶方法
1. 开盖前先离心,使样品集中于管底。
2. 用无菌蒸馏水复溶至 0.1–0.5 mg/mL,避免涡旋振荡或剧烈吹打。
3. 长期保存时,建议加入载体蛋白或稳定剂,例如 0.1% 牛血清白蛋白(BSA)、5% 人血清白蛋白(HSA)、10% 胎牛血清(FBS)或 5% 海藻糖。
4. 将复溶后的蛋白溶液分装保存,尽量减少冻融次数。
保存条件
在 −80~−20 ℃ 条件下可保存 12 个月。建议选择适宜的保存条件,并避免反复冻融。
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文献和实验Cytotoxicity, DNA Fragmentation, and DNA Repair Synthesis in Primary Human Hepatocytes
by metabolic activation/detoxification processes. Primary cultures of human hepatocytes that can be prepared from material discarded during the course of prescribed surgery offer the unique advantage of directly assessing the cytotoxic and genotoxic effects
Human chorionic gonadotropin (hCG) has been considered as a primary target molecule for a contraceptive vaccine by the World Health Organization because of its physiological and temporal specificity. hCG is an essential factor
The human mitochondrial genome encodes a variety of genes required for oxidative phosphorylation, and loss of these essential gene functions induces a multitude of severe metabolic disorders (1 –4 ). Mitochondrial DNA (mtDNA) is replicated
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