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文献和实验Characterization and Validation of Cre‐Driver Mouse Lines
are increasingly popular strategies in biomedical research. These approaches rely on the production of conditional genetically engineered mutant mouse (GEMM) lines with mutations in protein?encoding genes. These conditional GEMMs are then bred
Generation of Spatio‐Temporally Controlled Targeted Somatic Mutations in the Mouse
segment amplified from an endogenous mouse gene (myogenin) with the primer pair ADV28 and ADV30. Pu1, Pu3, and Pu4 are transgenic for K14‐Cre‐ERT2 ; Pu2 and Pu5 are not. View Image
describes vital staining of neuromuscular junctions in the mouse triangularis sterni muscle in one incubation step, combining presynaptic, motor nerve terminal staining with the styryl dye FM1?43, which labels recycling synaptic vesicles, and TRITC
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