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文献和实验Chromatin Immunoprecipitation Protocol for Histone Modification
we do so in large quantities. We would make a lysate from 7-10 maxi (15cm) dishes, sonicate the bulk chromatin lysate and store the preparation at 4°C. Naturally, the procedure remains the same for smaller scale ChIP experiments. Add 1/10th
-mediated pathways in the nucleus, Nature Reviews Genetics, 6(1): 24-35. 3.Morris, K.V. (2005) siRNA-mediated transcriptional gene silencing: the potential mechanism and a possible role in the histone code, Cell and Molecular Life Sciences, 62(24): 3057
, as it represents the percentage of immunoprecipitated chromatin. In a standard analysis of histone modifications, no more than 15% of the input native chromatin should be precipitated. However, this depends on the nature and the abundance of the histone
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