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- 技术资料
- CAS号：
20069-09-4
- 规格：
1mLx10mM(inDMSO)/5mg/10mg/25mg/50mg/100mg
| 规格： | 1mLx10mM(inDMSO) | 产品价格： | ￥215.0 |
|---|---|---|---|
| 规格： | 5mg | 产品价格： | ￥196.0 |
| 规格： | 10mg | 产品价格： | ￥330.0 |
| 规格： | 25mg | 产品价格： | ￥497.0 |
| 规格： | 50mg | 产品价格： | ￥757.0 |
| 规格： | 100mg | 产品价格： | ￥1136.0 |
Product Introduction
Bioactivity
| 名称 | Piperlongumine |
| 描述 | Piperlongumine (Piplartine) is an alkaloid natural product with anti-tumor, anti-inflammatory, anti-oxidant, anti-angiogenic and other biological activities. Piperlongumine enhances the level of ROS and induces apoptosis in tumor cells. |
| 细胞实验 | MCF-7 and 786-O cells are incubated with various PL concentrations for 48h. Cell proliferation is analysed by CellTiter Blue assay. Effective doses (ED) are calculated using XLift, Microsoft Excel add-in. (Only for Reference) |
| 激酶实验 | MEK Kinase Assay: Kinase inactive murine ERK2 (mERK2) K52A/T183A is affinity purified from Escherichia coli expressed using the pET21a vector. MEK1 kinase activity is determined using mERK2 K52A T183A as the substrate. Recombinant MEK1 enzyme (5 nM) is first activated by 0.02 unit or 1.5 nM of RAF1 in the presence of 25 mM HEPES (pH 7.8), 1 mM MgCl2, 50 mM NaCl, 0.2 mM EDTA, and 50 μM ATP for 30 minutes at 25 °C. The reactions are initiated by adding 2 μM of mERK2K52A T183A and 2.5 μCi [γ-33P] ATP in a total volume of 20 μL. The MEK2 kinase activity is determined similarly except that activation by RAF1 is not needed and 11 nM of MEK2 enzyme (active) are used in the assays.Kinase profiling is performed by Invitrogen using their Select Screen Kinase Profiling Service. The Z'-LYTE biochemical assay is used. RDEA119 is assayed in quadruplicate at 10 μM against 205 kinases. |
| 体外活性 | 方法：7 株人骨髓瘤细胞系用 Piperlongumine 处理 48 h，使用 CCK-8 assay 检测细胞活力。 结果：Piperlongumine 孵育 48 h 以剂量依赖的方式抑制 MM 细胞生长，IC50 值范围为 1-5 µM。[1] 方法：人乳腺癌细胞系 MCF-7 用 Piperlongumine (10-20 µM) 处理 24 h，使用 Flow cytometry 检测细胞周期。 结果：与对照组相比，G2/M 期的细胞分布显著增加了 5.56% (10 µM) 和 11.07% (20 µM)。Piperlongumine 在细胞周期中通过 G2/M 阻滞抑制细胞增殖。[2] |
| 体内活性 | 方法：为研究抗肿瘤活性，将 Piperlongumine (50 mg/kg，10% DMSO and 10% Tween 80 in water) 腹腔注射给携带 NCI-H929 异种移植物的裸鼠，每周五次，持续三周。 结果：用 Piperlongumine 治疗的小鼠显示出肿瘤生长延迟，cleaved caspase-3 水平增加，p-STAT3 水平降低。[1] |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (6.3 mM), Sonication is recommended. DMSO : 128.75 mg/mL (405.72 mM), Sonication is recommended. |
| 关键字 | ROS | ReactiveOxygenSpecies | Reactive Oxygen Species | Piperlongumine | Inhibitor | inhibit | Ferroptosis | Extracellular signal regulated kinases | ERK | Bacterial | Autophagy | Apoptosis |
| 相关产品 | Formamide | Guanidine hydrochloride | Aceglutamide | Neomycin sulfate | Hemin | Dimethyl sulfoxide | Sodium bicarbonate | Gluconate Calcium | Hyaluronic acid sodium (MW 20 kDa) | Sodium diacetate | D(+)-Raffinose pentahydrate | Ethyl linoleate |
| 相关库 | Anti-Cancer Active Compound Library | Bioactive Compounds Library Max | Selected Plant-Sourced Compound Library | Kinase Inhibitor Library | Food as Medicine Compound Library | Anti-Tumor Natural Product Library | Traditional Chinese Medicine Monomer Library | Cosmetic Ingredient Compound Library | Immunology/Inflammation Compound Library | Anti-Inflammatory Traditional Chinese Medicine Compound Library | Pain-Related Compound Library | Anti-infective Natural Product Library |
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￥196 - 1136
