- ￥191 - 1976
- TargetMol
- T3847
- 2026年07月07日
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- CAS号：
14259-45-1
- 规格：
1mg/5mg/10mg/25mg/50mg
| 规格： | 1mg | 产品价格： | ￥191.0 |
|---|---|---|---|
| 规格： | 5mg | 产品价格： | ￥444.0 |
| 规格： | 10mg | 产品价格： | ￥741.0 |
| 规格： | 25mg | 产品价格： | ￥1320.0 |
| 规格： | 50mg | 产品价格： | ￥1976.0 |
Product Introduction
Bioactivity
| 名称 | Asperuloside |
| 描述 | Asperuloside (Asperulosid) has anti-inflammatory activity. |
| 激酶实验 | The chemiluminescent assay is used to confirm PCSEE MAO-A and MAO-B inhibitory effects and to test BNN and BVN hMAO-A and hMAO-B inhibition using MAO-Glo kit. Each enzyme's Arbitrary Light Unit (ALU) is measured in the presence of PCSEE, BNN, BVN, and standard DEP as an MAO-BI positive control. Briefly, hMAO-A and hMAO-B isozymes are diluted to 2× with reaction buffer (pH 7.4) and preincubated with 4× PCSEE, BNN, BVN, or DEP working solutions at RT for 30?min in white opaque 96-well plates. For determining activity inhibition, final 8.5?μg/mL concentrations of PCSEE, BNN, BVN, and DEP are used. For IC50 determination, 8× PCSEE and BNN working solutions are serially diluted using reaction buffers (pH 7.4) to make a 4× concentration. Ten points' range of PCSEE (1.0 to 250.0?μg/mL) and BNN (up to 400?μM (135.4?μg/mL)) final concentrations is used. Controls used are with and without ethanol. Ethanol solvent in controls is kept to a maximum final (volume) of ≤2%. Each isozyme is substituted with the reaction buffer for the blank. Based on our preliminary optimizations and Valley's method, the reaction is initiated by adding 4× luciferin derivative substrate (LDS) for a final (concentration) of 40 and 4?μM for hMAO-A and hMAO-B reactions, respectively. The final volume per well of each reaction is 50?μL. The reaction is optimized for the amount of A and B enzyme used to be incubated for less than 3.5?h at RT. To stop the reaction and produce the luminescence signal RLDR is added to all wells, 50?μL to each well, and incubated for a further 30?min. |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (4.83 mM), Sonication is recommended. DMSO : 255 mg/mL (615.41 mM), Sonication is recommended. |
| 关键字 | NOSynthase | NOS | NO Synthase | Nitric oxide synthases | Inhibitor | inhibit | Asperuloside |
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| 相关库 | Bioactive Compound Library | Selected Plant-Sourced Compound Library | Heat-Clearing and Detoxifying Traditional Chinese Medicine Compound Library | Ancient Chinese Classical Formulas Compound Library | Miao medicine Compound Library | Food as Medicine Compound Library | Anti-Aging Compound Library | Traditional Chinese Medicine Monomer Library | Immunology/Inflammation Compound Library | Terpene Natural Product Library | Saccharide and Glycoside Natural Product Library | Natural Product Library for HTS |
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车叶草苷【14259-45-1】
￥191 - 1976
