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- 文献和实验
- 技术资料
- CAS号：
491-80-5
- 规格：
1mLx10mM(inDMSO)/100mg/200mg/500mg
| 规格： | 1mLx10mM(inDMSO) | 产品价格： | ￥256.0 |
|---|---|---|---|
| 规格： | 100mg | 产品价格： | ￥201.0 |
| 规格： | 200mg | 产品价格： | ￥268.0 |
| 规格： | 500mg | 产品价格： | ￥591.0 |
Product Introduction
Bioactivity
| 名称 | Biochanin A |
| 描述 | Biochanin A (4-Methylgenistein) is an isoflavone derivative isolated from red clover Trifolium pratense with anticarcinogenic properties. Biochanin A is a naturally occurring fatty acid amide hydrolase (FAAH) inhibitor. |
| 激酶实验 | For experiments with FAAH, rat liver homogenates, mouse brain homogenates and membranes from COS7 cells transfected with the human enzyme are used. Frozen (?80°C) livers from adult C57BL/6 mice and frozen brains (minus cerebella) from adult Wistar or Sprague-Dawley rats are thawed and homogenized in 20 mM HEPES, 1 mM MgCl2, pH 7. The homogenates are centrifuged at ~35000×g for 20 min at 4°C. After resuspension in buffer followed by recentrifugation and a second resuspension in buffer, the pellets are incubated at 37°C for 15 min. This incubation is undertaken in order to hydrolyse all endogenous FAAH substrates. The homogenates are then centrifuged as above, recentrifuged and resuspended in 50 mM Tris-HCl buffer, pH 7.4, containing 1 mM EDTA and 3 mM MgCl2. The homogenates are then frozen at ?80°C in aliquots until used for assay. FAAH is assayed in the homogenates and in the COS7 cell membranes using 0.5 μM (unless otherwise stated) [3H]AEA labelled in the ethanolamine part of the molecule. Blank values are obtained by the use of buffer rather than homogenate. In the experiments comparing effects of Biochanin A upon FAAH and FAAH-2, the same assay is used but with 16 nM [3H]oleoylethanolamide ([3H]OEA) as substrate and with an incubation phase at room temperature. The choice of OEA rather than AEA for FAAH-2 is motivated by the relative rates of hydrolysis: OEA is metabolized four times faster than AEA by FAAH-2, whereas for FAAH the rate of hydrolysis of OEA is about a third of that for AEA. When 0.5 μM [3H]AEA is used as substrate, assay conditions for rat brain and mouse liver are chosen so that <10% of added substrate is metabolized. For the human FAAH samples, <5% of the [3H]AEA is metabolized in all cases. For 16 nM [3H]OEA, a limited supply of an expensive ligand meant that optimization is not possible, and the amount of substrate utilized is higher (34±1 and 0.5±0.1% for FAAH and its corresponding mock-transfected, respectively; 40±2 and 21±0.4 for FAAH-2 and its corresponding mock-transfected respectively)[1]. |
| 体内活性 | LD50（小鼠）：63 mg/kg（腹腔注射）[4]。 |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | 10% DMSO+90% Saline : < 10 mg/mL (35.18 mM), Lower concentrations may be soluble, but exact solubility limit is unknown. 10% DMSO+40% PEG300+5% Tween 80+45% Saline : < 10 mg/mL (35.18 mM), Lower concentrations may be soluble, but exact solubility limit is unknown. DMSO : 250 mg/mL (879.48 mM), Sonication is recommended. 10% DMSO+90% Corn oil : 10 mg/mL (35.18 mM), Solution. Ethanol : 9 mg/mL (31.66 mM), Sonication is recommended. 10% DMSO+90% (20% SBE-β-CD in Saline) : < 10 mg/mL (35.18 mM), Lower concentrations may be soluble, but exact solubility limit is unknown. H2O : < 1 mg/mL (insoluble or slightly soluble) |
| 关键字 | Inhibitor | inhibit | Fatty acid amide hydrolase | FAAH (rat) | FAAH (mouse) | FAAH (human) | FAAH | EGFR | Biochanin A | Autophagy |
| 相关产品 | Guanidine hydrochloride | Glycerol | Aceglutamide | Malic acid | DL-Lysine | Sucrose | Hemin | Guanidine thiocyanate | Nicotinamide riboside malate | Gluconate Calcium | Thymidine | D(+)-Raffinose pentahydrate |
| 相关库 | Inhibitor Library | Bioactive Compound Library | Anti-Cancer Active Compound Library | Bioactive Compounds Library Max | Kinase Inhibitor Library | Food as Medicine Compound Library | Anti-Tumor Natural Product Library | Cosmetic Ingredient Compound Library | Natural Product Library | Membrane Protein-targeted Compound Library | Tyrosine Kinase Inhibitor Library | Anti-Inflammatory Traditional Chinese Medicine Compound Library |
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￥201 - 591
