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- 文献和实验
- 技术资料
- CAS号：
6990-06-3
- 规格：
1mLx10mM(inDMSO)/100mg
| 规格： | 1mLx10mM(inDMSO) | 产品价格： | ￥128.0 |
|---|---|---|---|
| 规格： | 100mg | 产品价格： | ￥128.0 |
Product Introduction
Bioactivity
| 名称 | Fusidic acid |
| 描述 | Fusidic acid (Fusidine) is an antibiotic isolated from the fermentation broth of Fusidium coccineum. |
| 激酶实验 | DHODase activity is measured by the DCIP colorimetric assay. This is a coupled assay in which oxidation of DHO and subsequent reduction of ubiquinone are stoichiometrically equivalent to the reduction of DCIP. Reduction of DCIP is accompanied by a loss of absorbance at 610 nm (ε=21500 M/cm). The assay is performed in a 96-well microtiter plate at ambient temperature (ca. 25°C). Stock solutions of 10 mM leflunomide and A771726 are prepared in dimethyl sulfoxide (DMSO) and these are diluted with reaction buffer (100 mM Tris and 0.1 % Triton X-100, pH 8.0) to prepare working stocks of the inhibitors at varying concentrations. For each reaction, the well contained 10 nM DHODase, 68 μM DCIP, 0.16 mg/mL gelatin, the stated concentration of ubiquinone, 10 μL of an inhibitor working stock to give the stated final concentration, and reaction buffer. After a 5-min equilibration period, the reaction is initiated by addition of DHO to the stated final concentrations. The total volume of reaction mixture for each assay is 150 μL, and the final DMSO concentration is ≤ 0.01% (v/v). The reaction progress is followed by recording the loss of absorbance at 610 nm over a 10-min period (during which the velocity remained linear). Velocities are reported as the change in absorbance at 610 nm per minute, and each reported value is the average of three replicates. In experiments where the DHO or ubiquinone concentration is varied, the other substrate is held constant at 200 μM. To determine the inhibitor potency of leflunomide and A771726, the effects of varying concentrations of the two compounds on the initial velocity of the DHODase reaction is measured over a concentration range of 0.01?1.0 μM. In these experiments the DHO and ubiquinone concentrations are held constant at 200 and 100 μM, respectively. |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | 10% DMSO+90% Saline : < 10 mg/mL (19.35 mM), Lower concentrations may be soluble, but exact solubility limit is unknown. 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 10 mg/mL (19.35 mM), Solution. DMSO : 125 mg/mL (241.92 mM), Sonication is recommended. |
| 关键字 | steroids | SQ-16603 | SQ16603 | SQ 16603 | ribosome | Inhibitor | inhibit | growth | Fusidic acid | Fusidate | factor | elongation factor G (EF-G) | elongation | CAT | bacteriostatic | Bacterial | Antibiotic |
| 相关产品 | Adipic dihydrazide | Levulinic acid | Doxycycline | Neomycin sulfate | Terbinafine hydrochloride | BES | Dimethyl sulfoxide | Sodium bicarbonate | Hyaluronic acid sodium (MW 20 kDa) | Sodium diacetate | Sulfamethoxazole sodium | D(+)-Raffinose pentahydrate |
| 相关库 | Microbial Natural Product Library | Bioactive Compound Library | Bioactive Compounds Library Max | ReFRAME Related Library | Natural Product Library | Immunology/Inflammation Compound Library | Drug Repurposing Compound Library | Terpene Natural Product Library | Natural Product Library for HTS | Pediatric Drug Library | Anti-infective Natural Product Library | Toxic Compound Library |
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夫西地酸【6990-06-3】
￥128
