- ￥1100 - 1880
- 上海经科
- 中国
- JK16158
- 2026年06月11日
- WB,IF,Flow Cyt,ELISA
- Mouse
- Human
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- 文献和实验
- 技术资料
- 免疫原：
POU domain class 5 transcription factor 1
- 亚型：
Mouse IgG
- 形态：
液体
- 保存条件：
-20℃/1year
- 克隆性：
Monoclonal
- 标记物：
non-conjugated
- 适应物种：
Human
- 保质期：
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- 抗原来源：
Oct-3/4 Monoclonal Antibody detects endogenous levels of Oct-3/4 protein.
- 目录编号：
WB 1:500-1:2000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
- 级别：
Affinity purification纯化
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 宿主：
Mouse
- 应用范围：
WB,IF,Flow Cyt,ELISA
- 浓度：
1mg/ml
- 靶点：
POU5F1
- 抗体英文名：
Oct-3/4 鼠单抗
- 抗体名：
POU5F1;OCT3;OCT4;OTF3;POU domain;class 5, transcription factor 1;Octamer-binding protein 3;Oct-3;Octamer-binding protein 4;Oct-4;Octamer-binding transcription factor 3;OTF-3
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1100.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1880.0 |
我们的优势
•国产平替产品：性价比极高
•全流程质控：原料溯源，每批都做WB/IHC/IF/ELISA验证
•亲和纯化：去除非特异性IgG，降低背景、杂带。
•批间一致性：内控标准严于国标，尽量保证批间差小。
•技术支持：博士团队、优化稀释比、修复条件、解决背景高/条带弱。
•抗体条带：清晰、杂带少、背景干净，稳定性好。
•组织染色：特异性强、定位准，复现性不错。
•国内现货：发货快。
•和多家三甲医院合作：组织样本经验足。
产品信息
| 产品名称 | Oct-3/4 Monoclonal Antibody WB,IF,Flow Cyt,ELISA |
| 产品货号 | JK16158 |
| 基因名称 | POU5F1 |
| 其他名称 | POU5F1;OCT3;OCT4;OTF3;POU domain;class 5, transcription factor 1;Octamer-binding protein 3;Oct-3;Octamer-binding protein 4;Oct-4;Octamer-binding transcription factor 3;OTF-3 |
| 宿主种属 | Mouse |
| 应用范围 | WB,IF,Flow Cyt,ELISA |
| 反应种属 | Human |
| 克隆性 | Monoclonal |
| 特异性 | Oct-3/4 Monoclonal Antibody detects endogenous levels of Oct-3/4 protein. |
| 同种型 | Mouse IgG |
| 保存液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| 纯化方式 | Affinity purification |
| 保存条件 | -20℃ |
| 稀释比例 | WB 1:500-1:2000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications. |
| 宿主种属 | Mouse |
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文献和实验This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013],
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
