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- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human Insulin Receptor/INSR/CD220 Protein
- 形态:
liquid
- 保存条件:
见COA
- 克隆性:
单克隆
- 保质期:
见COA
- 库存:
大量
- 供应商:
北京辰辉创聚生物技术有限公司
- 应用范围:
IHC-P
- 抗体英文名:
Anti-Insulin Receptor Monoclonal Antibody
- 抗体名:
抗Insulin Receptor单抗
- 规格:
100ul; 500ul; 1ml

| 产品信息
品名:NebuSelect™ Anti-Insulin Receptor Monoclonal Antibody
货号:NBAB-108068
品牌:辰辉创聚生物®️
规格:100ul; 500ul; 1ml
| 产品描述
NebuSelect™ Anti-Insulin Receptor Monoclonal Antibody (CAT# NBAB-108068) was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Insulin Receptor/INSR/CD220.
| 产品属性
Product Category:Mouse MAb
Reactivity:Human
Specificity:Human Insulin Receptor
Immunogen:Recombinant Human Insulin Receptor/INSR/CD220 Protein
Isotype:Monoclonal Mouse IgG1
Purification:Protein A
Buffer:0.2 μm filtered solution in PBS
Conjugate:Unconjugated
Applications:IHC-P
Dilution:IHC-P: 1:100-1:500
Storage:This liquid antibody can be stored at 2℃-8℃ for at least two weeks. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or antibody diluent, the antibody is stable for at least two weeks at 2-4℃.
For Research Use Only!
To get more information, please contact us freely.
郑重声明:仅用于科学研究,不可用于诊断或治疗。
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文献和实验Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates
This chapter describes techniques to successfully detect tyrosine phosphorylation of the insulin receptor and insulin receptor substrate proteins. These assays demonstrate whether the insulin signaling pathway is activated at its earliest
胰岛素受体是一个四聚体,由两个α亚基和两个β亚基通过二硫键连接。两个α亚基位于细胞质膜的外侧,其上有胰岛素的结合位点;两个β亚基是跨膜蛋白,起信号转导作用。无胰岛素结合时,受体的酪氨酸蛋白激酶没有活性。当胰岛素与受体的α亚基结合并改变了β亚基的构型后,酪氨酸蛋白激酶才被激活,激活后可催化两个反应∶①使四聚体复合物中β亚基特异位点的酪氨酸残基磷酸化,这种过程称为自我磷酸化(autophosphorylation);②将胰岛素受体底物(insulin receptor substrate
胰岛素受体底物(insulin receptor substrate,IRSs)
蛋白质,其上有多个(至少8个)可被受体激酶磷酸化的位点,磷酸化后可同多种效应物结合,包括:PI(3)K、Syp(一种磷酸酪氨酸磷酸酶)、Nck(一种连接蛋白)、GRB2(growth factor receptor-bound protein 2,一种通过SH2同磷酸化的酪氨酸结合的连接蛋白)。第二种是Shc(是通过cDNA克隆筛选到的编码SH结构域的基因的蛋白产物),也是一种连接蛋白。Shc的酪氨酸被磷酸化后能够同GRB2结合,然后激活Ras,触发细胞的增殖。第三种底物是IRS2。IRS2的酪氨酸被磷酸
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