DAB2 Polyclonal antibody

【产品概述】
本产品为DAB2 Polyclonal antibody（货号：10109-2-AP），由Rabbit / IgG宿主制备。该抗体属于Polyclonal抗体，可识别human, mouse, rat来源的靶蛋白，经测试适用于WB, IHC, IF/ICC, FC (Intra), IP, ELISA等实验应用。产品形式为Liquid，偶联物类型为Unconjugated。

【背景介绍】
DAB2 is a protein of 770 amino acid residues with a predicted molecular weight of 85.5kDa. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2-like domains, alpha and beta). Human DAB2 has an overall 83% identify with the mouse p96 protein, a putative mitogen-responsive phosphoprotein; homology is strongest in the amino-terminal end of the protein in a region corresponding to the phosphotyrosine interaction domain (PID), and contains multiple SH3 binding motifs. Chromosomal localization by FISH showed that the DAB2 gene is located on 5p13. The expression of DAB2 is down-regulated or absent in all the carcinoma cell lines examined, including prostate and ovarian carcinoma cell lines. The N-terminal domain of DAB2 interacts with Dishevelled-3 (Dvl-3), a signaling mediator of the Wnt pathway. Ectopic expression of DAB2 attenuates canonical Wnt/catenin-mediated signaling, including accumulation of catenin and cyclin D1 induction. DAB2 suppresses both protein kinase C and peptide growth factor-elicited signal pathways via the Ras-mitogen-activated protein kinase pathway. The proline-rich domain of DAB2 also interacts with proteins containing SH3 domain, such as Src and Fgr. The binding of DAB2 to c-Src resultes in the inactivation of c-Src. All data suggest that DAB2 is a potent tumor suppressor in many cancer types.

【产品特点】
本抗体采用Antigen affinity purification方式纯化，确保了高特异性和批次间一致性。观测分子量约为96 kDa。推荐稀释比：WB: WB : 1:500-1:2000；IHC: IHC : 1:50-1:500；IP: IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate。Western Blot检测阳性对照组织/细胞为HeLa cells, MCF-7 cells, RAW 264.7 cells, rat testis tissue。储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.。Proteintech抗体经过严格的质量验证流程，确保研究人员获得可重复且可靠的实验结果。

【产品信息】
GenBank蛋白编号：BC003064；基因名称：DAB2；Gene ID (NCBI)：1601；UNIPROT ID：P98082；RRID：AB_2089700；别名包括：Adaptor molecule disabled-2, Differentially expressed in ovarian carcinoma 2, Differentially-expressed protein 2, Disabled homolog 2, DOC 2；免疫原为CatNo:Ag0162Product name: Recombinant human DAB2 proteinSource:e coli.-derived, PGEX-4TTag: GSTDomain: 340-580 aa ofBC003064Sequence: VDYFGQQFDQISNRTGKQEAQAGPWPFSSSQTQPAVRTQNGVSEREQNGFSVKSSPNPFVGSPPKGLSIQNGVKQDLESSVQSSPHDSIAIIPPPQSTKPGRGRRTAKSSANDLLASDIFAPPVSEPSGQASPTGQPTALQPNPLDLFKTSAPAPVGPLVGLGGVTVTLPQAGPWNTASLVFNQSPSMAPGAMMGGQPSGFSQPVIFGTSPAVSGWNQPSPFAASTPPPVPVVWGPSASVA种属同源性预测；储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.；产品形式为Liquid；纯化方式为Antigen affinity purification。