- ￥300 - 3000
- proteintech
- 国内
- 21157-1-AP
- 2026年05月12日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit / IgG
- human, mouse, pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
详询
- 亚型：
IgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, pig
- 保质期：
详询
- 抗原来源：
详询
- 目录编号：
详询
- 级别：
科研级
- 库存：
现货
- 供应商：
上海艾科斯生物科技有限公司
- 宿主：
Rabbit / IgG
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 浓度：
400 ug/ml
- 靶点：
CXCL4/PF4
- 抗体英文名：
CXCL4/PF4
- 抗体名：
CXCL4/PF4
- 规格：
50 μL/100 μL/150 μL
【产品概述】
本产品为CXCL4/PF4 Polyclonal antibody（货号：21157-1-AP），由Rabbit / IgG宿主制备。该抗体属于Polyclonal抗体，可识别human, mouse, pig来源的靶蛋白，经测试适用于WB, IHC, IF/ICC, IP, ELISA等实验应用。产品形式为Liquid，偶联物类型为Unconjugated。
【背景介绍】
The chemokine (C-X-C motif) ligand 4 (CXCL4) is also named as PF4 (Platelet factor-4) and SCYB4. The platelets, the most abundant source of CXCL4 in vivo, control profibrotic macrophage activation via CXCL4. (PMID: 36807143). Exogenous CXCL4 drove profibrotic activation of monocyte-derived dendritic cells in vitro (PMID: 33042127). Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation.
【产品特点】
本抗体采用Antigen affinity purification方式纯化，确保了高特异性和批次间一致性。观测分子量约为10 kDa。推荐稀释比：WB: WB : 1:2000-1:10000；IHC: IHC : 1:50-1:500；IP: IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate。Western Blot检测阳性对照组织/细胞为Human peripheral blood platelets tissue, mouse spleen tissue。储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.。Proteintech抗体经过严格的质量验证流程，确保研究人员获得可重复且可靠的实验结果。
【产品信息】
GenBank蛋白编号：BC093965；基因名称：PF4；Gene ID (NCBI)：5196；UNIPROT ID：P02776；RRID：AB_2878819；别名包括：CXCL4, PF4, SCYB4, C X C motif chemokine 4, C-X-C motif chemokine 4；免疫原为CatNo:Ag12345Product name: Recombinant human PF4 proteinSource:e coli.-derived, PGEX-4TTag: GSTDomain: 1-101 aa ofBC093965Sequence: MSSAAGFCASRPGLLFLGLLLLPLVVAFASAEAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLES种属同源性预测；储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.；产品形式为Liquid；纯化方式为Antigen affinity purification。
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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