CD33 Polyclonal antibody

【产品概述】
本产品为CD33 Polyclonal antibody（货号：17425-1-AP），由Rabbit / IgG宿主制备。该抗体属于Polyclonal抗体，可识别human, mouse来源的靶蛋白，经测试适用于WB, IF/ICC, ELISA等实验应用。产品形式为Liquid，偶联物类型为Unconjugated。

【背景介绍】
CD33 (also known as sialic acid binding Ig-like lectin 3, or Siglec-3) is a transmembrane receptor expressed on cells of myeloid lineage ( It belongs to the Siglec family of sialic-acid-binding immunoglobulin-like lectins that are thought to promote cell-cell interactions and regulate the functions of cells in the innate and adaptive immune systems through glycan recognition. CD33 preferentially binds α2-6-linked sialic acids on lactosamine units. It is a member of the immunoglobulin superfamily of proteins and contains two immunoglobulin domains (one IgV and one IgC2 domain) responsible for ligand binding, a transmembrane region, and a cytoplasmic tail that contains two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that are implicated in the inhibition of cellular activity (PMID:10206955, PMID:10887109). What is the molecular weight of CD33? CD33 is a 364 amino acid glycoprotein that has an apparent molecular weight of 67 kDa by SDS-PAGE, with a predicted mass of 40 kDa based on the amino acid sequence. The disparity observed between the predicted and apparent molecular weight is due to the glycosylation of CD33 at five sites. What is the subcellular localization of CD33? CD33 is localized to the cell surface, but it can also be detected in the nucleus (PMID: 25613900). What is the tissue specificity of CD33? CD33 is expressed by myeloid stem cells (CFU-GEMM, CFU-GM, CFU-G, and E-BFU), myeloblasts and monoblasts, monocytes/macrophages, granulocyte precursors (with expression decreasing with maturation), and mast cells. Mature granulocytes may show a very low level of CD33 expression. It is not expressed on erythrocytes, platelets, B-cells, T-cells, or NK cells. The highest tissue expression of CD33 is observed in the spleen, bone marrow, lymph node, tonsil, and appendix (PMID: 25613900). What is the function of CD33 in myeloid cells? CD33 may act as an inhibitory receptor on myeloid cells to affect their function. U

【产品特点】
本抗体采用Antigen affinity purification方式纯化，确保了高特异性和批次间一致性。观测分子量约为67 kDa。推荐稀释比：WB: WB : 1:1000-1:6000。Western Blot检测阳性对照组织/细胞为EL-4-B5 cells, K-562 cells, THP-1 cells。储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.。Proteintech抗体经过严格的质量验证流程，确保研究人员获得可重复且可靠的实验结果。

【产品信息】
GenBank蛋白编号：BC028152；基因名称：CD33；Gene ID (NCBI)：945；UNIPROT ID：P20138；RRID：AB_2228962；别名包括：CD33 molecule, gp67, p67, Sialic acid-binding Ig-like lectin 3, SIGLEC 3；免疫原为CatNo:Ag11490Product name: Recombinant human CD33 proteinSource:e coli.-derived, PGEX-4TTag: GSTDomain: 49-259 aa ofBC028152Sequence: YYDKNSPVHGYWFREGAIISGDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVH种属同源性预测；储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.；产品形式为Liquid；纯化方式为Antigen affinity purification。