- ￥300 - 3000
- proteintech
- 国内
- 12573-1-AP
- 2026年05月11日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit / IgG
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
详询
- 亚型：
IgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详询
- 抗原来源：
详询
- 目录编号：
详询
- 供应商：
上海艾科斯生物科技有限公司
- 宿主：
Rabbit / IgG
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 浓度：
400 ug/ml
- 靶点：
TRIM25
- 抗体英文名：
TRIM25
- 抗体名：
TRIM25
- 规格：
50 μL/100 μL/150 μL
【产品概述】
本产品为TRIM25 Polyclonal antibody（货号：12573-1-AP），由Rabbit / IgG宿主制备。该抗体属于Polyclonal抗体，可识别human来源的靶蛋白，经测试适用于WB, IHC, IF/ICC, IP, ELISA等实验应用。产品形式为Liquid，偶联物类型为Unconjugated。
【背景介绍】
TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790). TRIM25 can be detected in both the cytoplasmic and nuclear (PMID: 33966039).
【产品特点】
本抗体采用Antigen affinity purification方式纯化，确保了高特异性和批次间一致性。观测分子量约为68-71 kDa。推荐稀释比：WB: WB : 1:5000-1:50000；IHC: IHC : 1:250-1:1000；IP: IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate。Western Blot检测阳性对照组织/细胞为HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells, HepG2 cells。储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.。Proteintech抗体经过严格的质量验证流程，确保研究人员获得可重复且可靠的实验结果。
【产品信息】
GenBank蛋白编号：BC016924；基因名称：TRIM25；Gene ID (NCBI)：7706；UNIPROT ID：Q14258；RRID：AB_2209732；别名包括：EC:2.3.2.27, EC:6.3.2.n3, EFP, Estrogen-responsive finger protein, RING-type E3 ubiquitin transferase；免疫原为CatNo:Ag3241Product name: Recombinant human TRIM25 proteinSource:e coli.-derived, PGEX-4TTag: GSTDomain: 298-630 aa ofBC016924Sequence: SLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQELTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGAPEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIKVILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFTEALYPAFWVFSAGATLSICSPK种属同源性预测；储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.；产品形式为Liquid；纯化方式为Antigen affinity purification。
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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