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- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
3 months at -20°C
- 英文名:
HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Tetramer, His & Avi, Human
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 规格:
100μg/500μg
| 规格: | 100μg | 产品价格: | ¥9900.0 |
|---|---|---|---|
| 规格: | 500μg | 产品价格: | ¥33000.0 |
| Species | Human | ||||||
| Protein Construction |
|
||||||
| Purity | > 95% as determined by BisTris PAGE | ||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 258 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
| Target Background | Survivin (also known as BIRC5) is an evolutionarily conserved eukaryotic protein that is essential for cell division and can inhibit cell death. Normally it is only expressed in actively proliferating cells, but is upregulated in most, if not all cancers; consequently, it has received significant attention as a potential oncotherapeutic target. |
| Synonyms | Survivin; MHC; MHC I; BIRC5; API4; EPR-1; IAP4; survivin variant 3 alpha |
产品特点:
1. 【人源天然序列】采用人源 HLA-A*02:01、B2M 天然氨基酸序列,结合 Survivin LMLGEFLKL 抗原肽,与体内天然 HLA 四聚体结构完全一致,可特异性结合 T 细胞受体2. 【高活性高特异性】可特异性结合对应 T 细胞受体,ED₅₀ < 100 ng/mL(T 细胞结合实验),可被 HLA 特异性抗体识别,适用于 T 细胞筛选、抗原特异性免疫研究3. 【高纯度低内毒素】SDS-PAGE 纯度 > 95%,内毒素 < 0.5 EU/μg(LAL 法),无支原体、细菌、真菌等微生物污染,适配多种科研实验需求4. 【双标签易纯化检测】哺乳动物细胞表达,N 端带 Avi 标签、C 端带 His 标签,可通过 Ni-NTA 亲和层析快速纯化,也可通过 Avi/His 标签抗体进行特异性检测5. 【稳定易保存】蛋白储存液配方优化,未开封 - 80℃可稳定保存 12 个月,-20℃可存 6 个月,避免反复冻融可长期保持蛋白活性6. 【多场景适配】可用于 HLA 蛋白结构与功能研究、Survivin 抗原特异性 T 细胞免疫机制研究、肿瘤免疫治疗研究、细胞培养添加剂、药物筛选、抗体研发等
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