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- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
3 months at -20°C
- 英文名:
IFN gamma/IFNG, His, Cynomolgus
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 规格:
100μg/500μg
| 规格: | 100μg | 产品价格: | ¥3500.0 |
|---|---|---|---|
| 规格: | 500μg | 产品价格: | ¥11500.0 |
| Species | Cynomolgus | ||||
| Protein Construction |
|
||||
| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 17.72 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
| Target Background | IFN-gamma and one of its receptor subunits, IFNGR1, are translocated to the nucleus, together with STAT1alpha as one macromolecular complex, via the classical importin-dependent pathway.Put IFN-gamma and its receptor subunit, IFNGR1, in direct contact with the promoter region of IFN-gamma-activated genes with associated increased activity, thus suggesting a transcriptional/cotranscriptional role for IFN-gamma/IFNGR1 as well as a possible role in determining the specificity of IFN-gamma action. |
| Synonyms | IFN-gamma-R1; CDw119; CD119; IFNGR1; IFNGR; AVP; FLJ45734; IFN-gamma R1; IFN-gR1; type 2; IFNγ; IFN γ |
产品特点:
1. 【食蟹猴源天然序列】采用食蟹猴 IFN-γ(IFNG)天然氨基酸序列,无突变,与食蟹猴体内天然蛋白结构完全一致,适配食蟹猴来源细胞的实验需求,无种属差异2. 【高活性高特异性】可高效结合 IFN-γ 受体,ED₅₀ < 100 ng/mL(受体结合实验),可被 IFN-γ 特异性抗体识别,适用于抗体筛选、免疫机制研究3. 【高纯度低内毒素】SDS-PAGE 纯度 > 95%,内毒素 < 0.5 EU/μg(LAL 法),无支原体、细菌、真菌等微生物污染,适配多种科研实验需求4. 【His 标签易纯化检测】哺乳动物细胞表达,C 端带 His 标签,可通过 Ni-NTA 亲和层析快速纯化,也可通过 His 标签抗体进行特异性检测5. 【稳定易保存】蛋白储存液配方优化,未开封 - 80℃可稳定保存 12 个月,-20℃可存 6 个月,避免反复冻融可长期保持蛋白活性6. 【多场景适配】可用于 IFN-γ 蛋白结构与功能研究、免疫调控与炎症机制研究、肿瘤免疫治疗研究、非人灵长类疾病模型研究、细胞培养添加剂、药物筛选、抗体研发等
IFN-γ,IFNG, 干扰素 γ, 食蟹猴源,食蟹猴,重组蛋白,重组,高活性,高纯度,低内毒素,His 标签,细胞培养,免疫细胞,科研实验,哺乳动物细胞表达,Cynomolgus,IFN-gamma,IFNG,Recombinant protein,Interferon gamma,His-tagged,High activity,High purity,Low endotoxin,Cell culture,Immune cell,Mammalian expression
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文献和实验cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.Sequence:MCPLRSLLLI STLVLLHHLP HLSLGRSLPT TTASPGRSCL DYSQNLLRAVSNTLQKARQT LEFYSCTSEE IDHEDITKDK TSTVEACLPL
的佐剂效应,对人体无不良反应。近期正在研究NDV细胞瘤苗结合双功能抗体(抗NDV上的凝血素神经氨酶HN x CD28、HN x CD3)的抗肿瘤效果。同期,我国钱振超教授应用NDV细胞瘤苗同时加入中药莪术提取物 b-榄香烯处理,动物实验有明显抗肿瘤效果,临床应用也取得了一定疗效。近些年转基因细胞瘤苗研究较多,其中转导的基因包括:IL-1、IL-2、IL-4、IL-6、IL-7、IL-12、TNF-a、G-CSF、GM-CSF、IFN-g基因;共刺激分子(B7-1、B7-2、)基因;同种异体抗原
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