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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
3 months at -20°C
- 英文名:
B7-H5/Gi24/VISTA[Biotin], His & Avi, Human
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 规格:
100μg/500μg
| 规格: | 100μg | 产品价格: | ¥5800.0 |
|---|---|---|---|
| 规格: | 500μg | 产品价格: | ¥19200.0 |
| Species | Human | ||||||
| Protein Construction |
|
||||||
| Conjugate | Biotin | ||||||
| Purity | > 95% as determined by BisTris PAGE | ||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 21.04 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
| Target Background | B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. |
| Synonyms | B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1 |
产品特点:
1. 【人源天然序列】采用人源 B7-H5(Gi24/VISTA)天然氨基酸序列,无突变,与体内天然蛋白结构完全一致,可特异性结合 B7-H5 配体2. 【高活性高特异性】可特异性结合 B7-H5 抗体,ED₅₀ < 100 ng/mL(抗体结合实验),可被 B7-H5 特异性抗体识别,适用于抗体筛选、免疫机制研究3. 【高纯度低内毒素】SDS-PAGE 纯度 > 95%,内毒素 < 0.2 EU/μg(LAL 法),适配免疫细胞培养、体内免疫实验等敏感场景需求4. 【多标签易纯化检测】哺乳动物细胞表达,N 端带 Avi 标签、C 端带 His 标签,可通过 Ni-NTA 亲和层析快速纯化,也可通过 Avi/His 标签抗体、生物素亲和素系统进行特异性检测5. 【稳定易保存】蛋白储存液配方优化,未开封 - 80℃可稳定保存 12 个月,-20℃可存 6 个月,避免反复冻融可长期保持蛋白活性6. 【多场景适配】可用于 B7-H5 蛋白结构与功能研究、免疫调控与免疫耐受机制研究、肿瘤免疫治疗研究、细胞培养添加剂、药物筛选、抗体研发等
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