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- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
3 months at -20°C
- 英文名:
IL-15RA&IL-15 [Biotin], His, Human
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 规格:
100μg/500μg
| 规格: | 100μg | 产品价格: | ¥5800.0 |
|---|---|---|---|
| 规格: | 500μg | 产品价格: | ¥19200.0 |
| Species | Human | ||||
| Protein Construction |
|
||||
| Conjugate | Biotin | ||||
| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1 EU per ug by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 23.8 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 25-28 kDa&30-35 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
| Target Background | Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development. |
| Synonyms | IL-15 R alpha;CD215;IL15RA;IL-15RA;IL-15R-alpha1;Interleukin-15;IL-15;IL15; Nanrilkefusp alfa |
产品特点:
1. 【人源天然序列】采用人源 IL-15RA、IL-15 天然氨基酸序列,无突变,与体内天然蛋白结构完全一致,可特异性结合 IL-15 受体2. 【高活性高特异性】可高效促进免疫细胞增殖,ED₅₀ < 100 ng/mL(细胞增殖实验),可被 IL-15 特异性抗体识别,适用于抗体筛选、炎症免疫机制研究3. 【高纯度低内毒素】SDS-PAGE 纯度 > 95%,内毒素 < 0.2 EU/μg(LAL 法),适配免疫细胞培养、体内免疫实验等敏感场景需求4. 【双标签易纯化检测】C 端带 His 标签,可实现生物素标记,可通过 Ni-NTA 亲和层析快速纯化,也可通过标签抗体、生物素亲和素系统进行特异性检测5. 【稳定易保存】蛋白储存液配方优化,未开封 - 80℃可稳定保存 12 个月,-20℃可存 6 个月,避免反复冻融可长期保持蛋白活性6. 【多场景适配】可用于 IL-15 蛋白结构与功能研究、炎症与免疫调控机制研究、肿瘤免疫治疗研究、抗体制备与筛选、药物靶点验证、细胞培养添加剂等
IL-15RA,IL-15, 人源,人,重组蛋白,重组,高活性,高纯度,低内毒素,生物素标签,His 标签,炎症免疫,细胞培养,免疫细胞,科研实验,哺乳动物细胞表达 Human,IL-15RA,IL-15,Recombinant protein,Biotin-tagged,His-tagged,High activity,High purity,Low endotoxin,Inflammatory immunity,Cell culture,Immune cell,Mammalian expression
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文献和实验Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.
Preparation and purification of biotin-labeled probe for ISH
product. The best ISH results are obtained when the size of the biotin-labeled probe is around 300-600 bp. To determine the size of the probe, denature 15 µl of the nick translation product in boiling water for 4 min and cool down on ice immediately
of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
与开发。 菲恩生物提供高纯度、高活性的IL-26重组蛋白,助力靶向IL-26通路的治疗药物的开发与筛选。 货号 产品名称 表达宿主 预测分子量 P6750 Recombinant Human IL-26 E.Coli 16.4 kDa 货号 英文名 灵敏度 检查范围 EH1888 Human IL26 (Interleukin-26) ELISA Kit 9.375pg/ml 15.625-1000pg/ml
技术资料

![重组蛋白 IL-15RA & IL-15 [Biotin] 人 His Biotin产品图](https://img1.dxycdn.com/p/s14/2026/0908/418/6583362937953417902.jpg)



![重组蛋白 Siglec-5/CD170 hFc Chimera [Biotin] 人 hFc Biotin](https://img1.dxycdn.com/p/s14/2026/0615/574/8617863096494236602.png!wh200)



