pitstop 2

Pitstop 2 is a clathrin inhibitor that inhibits clathrin-mediated endocytosis (CME) by binding to the terminal structural domain of clathrin¹².At a concentration of 20-40 μM for 30 minutes, pitstop 2 can inhibit the endocytosis of transferrin by J774A.1 macrophages without damaging cell viability or affecting cholera toxin B internalization in J774A.1 macrophages¹.At concentrations less than 100 μM for 6 hours, pitstop 2 inhibits the mitotic spindle and affects mitotic progression in HeLa cells. In the concentration range of 1-30 μM, pitstop 2 induced apoptosis in dividing cancer cells and inhibited cell growth at 24 h. At 48 h, pitstop 2 inhibited the mitotic spindle and affected the mitotic progression of HeLa cells. At 48 hours of action, pitstop 2 did not affect the growth and viability of non-carcinogenic NIH3T3 fibroblasts².

溶解方案(细胞实验)

DMSO 中的溶解度 : 50 mg/mL (105.63 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

溶解方案(动物实验)

"方案 一": "请依序添加每种溶剂：10% DMSO 40% PEG300 5% Tween-80 45% SalineSolubility: ≥ 2.5 mg/mL (5.28 mM); 澄清溶液 此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。"