OSMI-4

O-linked N-acetylglucosamine (OGlcNAc) transferase (OGT) is an essential enzyme responsible for nucleocytoplasmic glycosylation. OSMI-4 is a potent OGT inhibitor with an EC50 value of ~3μM. Treatment of HEK293T cell lysates with OSMI-4 for 24 hours almost completely reduced the level of OGlcNAc at a concentration of 5μM. Cells treated with 20μM of OSMI-4 for 48 hours also showed decreased levels of HCF-1 cleavage products and the appearance of uncleaved HCF-1 compared to the control group. Also in HEK293T cells, 2-hour treatment with 10μM of OSMI-4 resulted in an increase in OGT’s detained intron splicing².

溶解方案(细胞实验)

DMSO 中的溶解度 : 62.5 mg/mL (103.46 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

溶解方案(动物实验)

"方案 一": "请依序添加每种溶剂：10% DMSO 40% PEG300 5% Tween-80 45% SalineSolubility: ≥ 2.08 mg/mL (3.44 mM); 澄清溶液 此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。"

"方案 二": "请依序添加每种溶剂：10% DMSO 90% Corn OilSolubility: ≥ 2.08 mg/mL (3.44 mM); 澄清溶液 此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。"