兔抗双链DNA抗体IgM(dsDNA)酶联免疫吸附检测试剂盒
【Rabbit dsDNA(anti-double stranded DNA antibody IgM) ELISA Kit】- ￥1960 - 2800
- ELK Biotechnology
- 1.57-100 ng/mL
- 0.6 ng/mL
- 武汉
- ELK9778
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
1.57-100 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Rabbit
- 样本：
serum, plasma, urine and other biological fluids
- 灵敏度：
0.6 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 兔抗双链DNA抗体IgM(dsDNA)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rabbit dsDNA(anti-double stranded DNA antibody IgM) ELISA Kit |
| 别名 | dsDNA Ab IgM |
| 货号 | ELK9778 |
| 反应种属 | Rabbit |
| 检测类型 | Sandwich |
| 灵敏度 | 0.6 ng/mL |
| 标准品 | 100 ng/mL |
| 检测范围 | 1.57-100 ng/mL |
| 样本类型 | serum, plasma, urine and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit dsDNA IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit dsDNA IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit dsDNA IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit dsDNA IgM in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Disease |
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文献和实验Double-stranded DNA (dsDNA) viruses of plants are believed to be plant pararetroviruses. Their genome is replicated by reverse transcription of a larger than unit-length terminally redundant RNA transcript of the viral genomic DNA using
Preparation of Single-Stranded Antisense cDNA Probes by Asymmetric PCR
, but they can generally be described as three types, namely double-stranded DNA (dsDNA) generated by random primed or symmetric PCR methods, riboprobes by RNA polymerase generation of antisense copy from target cDNA, and, more recently, antisense single-stranded DNA
Site-Directed Mutagenesis of Antibody-Variable Regions
strands using single-stranded phage or phagemid DNA as template. The original strand is removed by exonuclease digestion, the nonmutant strand is nicked with a restriction enzyme, gapped with exonuclease III, and mutant double-stranded DNA is generated
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