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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates and other biological fluids.
- 灵敏度:
0.063 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 人5-羟色胺受体6(5HTR6)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human 5HTR6(5-Hydroxytryptamine Receptor 6) Microsample ELISA Kit |
| 别名 | 5-HT6; 5-HT6R; Serotonin Receptor 6; 5-HT6 Receptor |
| 货号 | ELK8828MS |
| 反应种属 | Human |
| P50406 | P50406 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.063 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 5HTR6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5HTR6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 5HTR6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5HTR6 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Neuro science; |

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文献和实验缩写 全称 中文 AA autoantibody 自身抗体 AAF aminoacetylfluorescence 氨乙酰基荧光 Ab antibody 抗体 ABC avidin-biotin peroxidase complex 抗生物素(亲合素)-生物素-过氧化物酶复合物 ACh acetycholine 乙酰胆碱 AChR acetycholine receptor 乙酰胆碱受体 AChRAb acetycholine
”分析MAPK磷酸化蛋白和总蛋白,以二者的比值评价MAPK的磷酸化状态。结果表明,人resistin可通过活化ERK 1/2、p38信号转导途径而诱导HCAEC增殖和迁移,提示人resistin可能在与血管发生有关的血管疾病中发挥作用。Sakai[20]为研究缺失突变的EGFR(表皮生长因子受体)的生物学作用,将转染EGFR的293细胞、人非小细胞肺癌细胞系A549、PC 9细胞在加入EGF培养后,取细胞裂解液用上述磷酸化蛋白检测试剂盒定量分析EGFR、p44/42 MAPK、p38 MAPK
,8:81-89 [20]Simmons T,Newhous Y M, Arnold K S et al . Human low density lipoprotein receptor fragment successful refolding of a functionally active ligand-binding domain produced in E.coli.J Biol Chem,1997,272(41):25531-25536 [21]Rozema D,Gellman
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