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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates and other biological fluids.
- 灵敏度:
0.065 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 人5-羟色胺受体6(5HTR6)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human 5HTR6(5-Hydroxytryptamine Receptor 6) ELISA Kit |
| 别名 | 5-HT6; 5-HT6R; Serotonin Receptor 6; 5-HT6 Receptor |
| 货号 | ELK8828 |
| 反应种属 | Human |
| P50406 | P50406 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.065 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 5HTR6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5HTR6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 5HTR6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5HTR6 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Neuro science; |

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文献和实验当您在研究用药物、细胞因子、抑制剂、病毒等作用细胞后细胞因子、信号通路(磷酸化)分子、免疫受体分子、细胞凋亡分子、干细胞标记分子、血管生成标记分子、肥胖症标记分子相关等蛋白分子表达变化的高通量初步筛选与定量检测时,面对大规模的WB实验,您是否烦躁了呢?面对表达量很低的蛋白,转膜造成的蛋白的丢失,您是否困惑啦?有一种方法可以解决上面的问题。它不需要特殊仪器,6小时最多能检测59种蛋白分子。它比WB的灵敏度高100倍左右;是WB方法和Elisa方法的融合,更有创新,比这两种方法更简单。它就是R&D
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