- ￥1960 - 2800
- ELK Biotechnology
- 3.13-200 pg/mL
- 0.15 pg/mL
- 武汉
- ELK080ES
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
3.13-200 pg/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Serum, plasma, cell culture supernatants
- 灵敏度：
0.15 pg/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 一步法人白介素5(IL5)酶联免疫吸附检测试剂盒 |
| 英文名称 | EasyStep Human IL5(Interleukin 5) ELISA Kit |
| 别名 | IL-5; E-CSF; ESGF; EDF; TRF; Colony-Stimulating Factor,Eosinophil; T-Cell replacing Factor; B Cell Differentiation Factor I; Eosinophil Differentiation Factor |
| 货号 | ELK080ES |
| 反应种属 | Human |
| 检测类型 | Sandwich |
| 灵敏度 | 0.15 pg/mL |
| 标准品 | 200 pg/mL |
| 检测范围 | 3.13-200 pg/mL |
| 样本类型 | Serum, plasma, cell culture supernatants |
| 反应时间 | 1.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL5, and the Human IL5 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL5. After TMB substrate solution is added, only those wells that contain Human IL5 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL5 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cytokine;Infection immunity; |
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文献和实验【讨论】Innate immunity: 'Natural helper' cells identified
. These structures were also found in the adipose tissues around the kidney and genitalia, as well as in the human mesentery, and were termed fat-associated lymphoid clusters (FALCs). Within these structures, KIT+SCA1+ cells were identified that do not express
的酶活力直接反映游离的酶标记物。均相EIA在临床检验中较少应用。非均相EIA需先进行游离的和结合的标记物的分离。如前所述，固相载体可用作一种分离手段。这种固相酶免疫测定方法在1971年最初建立时称为酶联免疫吸附剂测定（enzyme linked immunosorbent assay），简称ELISA，在国内有译作酶联免疫吸附试验或酶标，已习用。2、ELISA的原理和类型2.1. ELISA的原理ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫
造血细胞因子IL3，IL5和GMCSF能引起嗜酸性粒细胞中黏附分子ICAM-1的表达，但是关于IL3，IL5和GMCSF介导的嗜酸性粒细胞表达并释放多种黏附分子的具体机制尚未阐明。 实验使用从新鲜人白血球层分离培养的嗜酸性粒细胞，在不同条件处理后，抽提RNA与SuperArray的人NF-κB信号通路基因芯片(GEArray Q series Human NF-κB Signaling Pathway Gene Array, Catalog No. HS-016N)杂交。实验结果分析发现在IL5
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