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- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.79-50 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma and other biological fluids
- 灵敏度:
0.28 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 人杀菌肽LL-37(LL-37)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human LL-37(Antibacterial Protein LL-37) ELISA Kit |
| 别名 | CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic; CAMP; Cathelicidin Antimicrobial Peptide |
| 货号 | ELK8189 |
| 反应种属 | Human |
| 检测类型 | Sandwich |
| 灵敏度 | 0.28 ng/mL |
| 标准品 | 50 ng/mL |
| 检测范围 | 0.79-50 ng/mL |
| 样本类型 | serum, plasma and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Infection immunity; |

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文献和实验在目前的以HRP作为标记酶的商品ELISA试剂盒中,如以TMB为底物,则提供的底物为A和B两瓶应用液;如以OPD为底物,则试剂盒提供OPD片剂或粉剂,临用前配制。一般商品试剂盒显色反应条件为37℃或室温反应15~30min。从理论上说,37℃30min才可以使HRP的底物催化反应完全,尽管在最初的10min内,绝大部分催化反应即可完成。因此,为使弱阳性样本孔能有充分的显色,建议在37℃下反应25~30min后,终止反应比色测定。此外,在加入底物开始显色反应前,最好是先检查一下底物溶液的有效
or Costar serocluster "U" vinyl plates #2797). Incubate for 4 hours at 37°C and then store at 4°C until use (if less than two weeks). For the vinyl plates, wash three times with washing solution, dispel liquid by slapping on paper towels and then cover
• 洗板一定力求干净,以保证实验成功。 图 9-1 免疫酶联吸附实验( ELISA )的实验原理 图 9-2 定性 ELISA 实验结果,左为阳性结果,右为阴性结果。
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