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- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
1.57-100 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Cattle
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.61 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 牛70kDa热休克蛋白1A(HSPA1A)酶联免疫吸附检测试剂盒 |
| 英文名称 | Cattle HSPA1A(Heat Shock 70kDa Protein 1A) ELISA Kit |
| 别名 | HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70 |
| 货号 | ELK8002 |
| 反应种属 | Cattle |
| Q27975 | Q27975 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.61 ng/mL |
| 标准品 | 100 ng/mL |
| 检测范围 | 1.57-100 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle HSPA1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HSPA1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle HSPA1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HSPA1A in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Tumor immunity;Infection immunity;Developmental science; |

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文献和实验Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA
The enzyme-linked immunosorbent assay (ELISA) is an immunological technique which is used to determine the presence or quantity of an antigen within a sample. ELISAs rely on the use of at least one antibody (Ab) specific for the antigen
65-KDa蛋白抗原的基因序列:结核杆菌65-KDa蛋白抗原为存在于所有分枝杆菌细胞壁中的热休克蛋白,也是一种交叉反应蛋白.所以依据该序列设计合成的引物,PCR能扩增出所有分枝杆菌的靶DNA片段,没有属内特异性.这种PCR较适用于结核病高发区大规模筛选和流行病普查.另外,对65-KDa蛋白基因的PCR扩增产物进行限制性酶切分析(RFLP),也可以准确地检测出结核杆菌的属内归属. 2.编码MPB64蛋白的基因序列:MPB64蛋白为结核杆菌复合群(MTBC,包括人型结核杆菌、牛型结核
序列 1.编码65-KDa蛋白抗原的基因序列:结核杆菌65-KDa蛋白抗原为存在于所有分枝杆菌细胞壁中的热休克蛋白,也是一种交叉反应蛋白.所以依据该序列设计合成的引物,PCR 能扩增出所有分枝杆菌的靶DNA片段,没有属内特异性.这种PCR 较适用于结核病高发区大规模筛选和流行病普查.另外,对65-KDa蛋白基因的PCR 扩增产物进行限制性酶切分析(RFLP),也可以准确地检测出结核杆菌的属内归属. 2.编码MPB64蛋白的基因序列:MPB64蛋白为结核杆菌复合群(MTBC,包括人型结核杆菌、牛
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